CHEUNG Tung Po

• Preparation of over 200 batches of competent B. subtilis cells
• Carrying out ad-hoc lab duties for other experiments
• Participating as an actor in promotion video
• Conducting preliminary market and competitor analysis during the ideation stage

CHOY Wing Hei

• Project Administration - Organised and led many IHP events, including podcast recordings, interviews with business owners and professors, creating Synthetic Sunday stories, co-hosting iGBA in Zhuhai, holding the iGEM Booth and writing up the IHP wiki pages.
• Public engagement - Conducted surveys and interviewed business owners to collect their views on b. cereus contamination, and raise public awareness toward cereulide poisoning at the same time. Recorded 4 podcast episodes to provide entertainment and educational content about synthetic biology for the public. Published Synthetic Sunday stories to spread interesting news about synthetic biology.
• Visualisation: Designed the project poster, infographics of our project findings and data research, as well as creating Instagram stories and posts for our team. Participated in the production of the promotion video as a scriptwriter and actor.
• Writing - Writing up the IHP wiki pages, podcast scripts, survey findings and data storytelling of cereulide cases worldwide.

FUNG Ching Nam

• Performing initial trials for potassium ion efflux, biofilm experiments
• Assist in interlab experiments
• Assist in data analysis of experiments
• Preliminary research on choice of chassis, sensing mechanism, experimental design
• Propose utilizing the two-component system in B. subtilis as the sensing module of the circuit

JOSH Bennet Tang

• Room Bookings for General Meetings
• Update Team and Advisors about Internal Changes (Membership Changes, Project Direction Changes, etc.)
• Helped research methodologies for Model creation and Sobol Analysis
• Helped Research Wiki Design Ideas

KIM Hyejun

• Performed experiment 1: biofilm formation
• Write script for the presentation video
• Assisted in researching hydrophobic modification of hydrogel for the biosensor
• Assisted in hardware experiments

LAU Hoi Ying

• Constructing Circuit Model, sensitivity analysis and model fitting with preliminary research on phosphorelay kinetics, kinetics of potassium efflux due to K+ ionophore and circuit components
• Side projects: Cereulide distribution - Image processing while assisting in Cereulide distribution model (Coding & Experimental design) & assist of Hardware app
• Proposed modeling cereulide production with temperature cooling effect versus time and assisting in Cereulide Production model (Research) Circuit design
• Assisting in the design of primers for testing out the degradation tag library and assisting in data analysis of experiments

LAI Chak Long

• Prepared competent B.subtilis and involved following specific protocols and ensuring the cells were competent
• Involved in designing, performing, troubleshooting, analyzing and writing wiki contents for Potassium ion Efflux Experiment

MOK Ngai Tang

• Performing the potassium ion efflux experiment and troubleshooting failed experiments
• Conducting preliminary market and competitor analysis
• Researching on potential stakeholders
• Conceptualizing the promotional and presentation video
• Documenting The 1st iGEM Greater Bay Area Synthetic Biology Industry–Academia-Research Forum in Zhuhai, China

PANG Chun Pan

• Performing 3D protein modelling for degradation tag library
• Coding for wiki pages using basic computer language(html+css, Javascript)
• Conducting time-point experiment for degradation tag library
• Conducting all interlab experiments

Rakshita RUPANI

• Conducted Experiment 1 and troubleshooted the experiment numerous times throughout the summer by testing with different culture media, incubation periods, dilutions, etc.
• Initiated Synthetic Sunday (weekly updates of synthetic biology news) and wrote the content for 7+ weeks.
• Volunteered at organizations such as Food Angel in order to learn more about their awareness and proactiveness against cereulide.
• Reached out to 50+ schools to see if they were interested in participating in our series.

SO Lok Yee

TEMIRBEKOV Yerassyl

• Proposal for cereulide distribution, development of sub-project
• Experiment design for cereulide distribution modeling (data acquisition method, 3d modeling of experimental setup)
• Initial versions of visualization and data processing software

YEUNG Sen

• Coordinate the Zhuhai, China, venue for the first iGEM Greater Bay Area Synthetic Biology Industry-Academic-Research Forum.
• Conduct all time point experiments on circuit
• Analysis data of experiments
• Conducted all interlab experiments
• Assist in data analysis of experiments

WONG Chun Fung

• Conducted preliminary research on award requirements, past iGEM team achievements, public awareness of cereulide poisoning, and organized meetings with other teams.
• Conducted preliminary research on B.cereus and cereulide production, also participated in biofilm experiment
• Took on administrative responsibilities

WONG Kei Hong

• Proposing the idea of the team project
• Performing the potassium ion efflux experiment and troubleshooting failed experiments for more than 15 times.
• Writing wiki content with more than 2000 words for potassium efflux experiment, B subtilis competent cell preparation and general protocols.
• Conducting preliminary market and competitor analysis during the ideation stage
• Researching on the project problem

WONG Nga Man

• Designing user manual and preparing the Test Kit Package for on-site detection of cereulide
• Preparing materials for experiments including chemicals, cell cultures and plates etc.
• Fine-tuned Protocol for B.Subtilis Gel Based Biosensor production, including the scale and material of gel, as well as cell culture and performance optimization
• Conduct several time-points experiments to inspect cells’ and spores’ sensing ability in LB and hydrogel using the plate reader
• Analyzing and visualizing data collected on plate reader, proving that PVA-PEG gel could enhance the performance of engineered B.Subtilis in terms of fluorescence signal output and processing efficiency

WONG Zhiping

• Coordinate the 1st iGEM Greater Bay Area Synthetic Biology Industry–Academia-Research Forum in Zhuhai, China
• Designed and source team souvenirs eg stickers, keychains and team wear
• Organise the production, produced animation, assist with filming, wrote script and provided chinese version of subtitle for promotional video
• Managed our team Instagram page
• Conduct interlab experiments

ZHENGLOV David

• Research on the potassium efflux model
• Coding for the potassium efflux model (kinetics of valinomycin-mediated K+ ion transport + overall rate of change of [K+])
• Building cereulide production model (coding, research and fitting data)
• Analyzed data for the cereulide production model