Safety

Safe project design

Our goal of the project is to address global microplastic pollution problem by engineer a whole-cell biosensors based on LasI-LasR quorum sensing regulatory circuit in P. aeruginosa to quantitatively detect and monitor microplastics in samples of an area, therefore offers easier identification and tracking of microplastics. This approach is important for policy formulation and prioritizes the allocated resources to address the issues.

Safe organisms

Our team selects non-pathogenic classis for usage. They are safe strains of Escherichia coli called DH5α and C41(DE3) to transform recombinant plasmids - pET21b (+), pET23b(+), pUC57 and pSB1C3 which carries the protein-coding sequences to produce the proteins. Both of the E.coli strains that we choose have no harm on humans nor the natural environment. There is no genetically engineered bacteria released into the environment.

Safe lab

All organisms involved in our experiments pose no serious risks even if escaped from the lab. No hazardous chemicals are used in our experiment. We utilized antiseptic techniques and used Bunsen burner to maintain sterility of the workplace and experimental apparatuses. Our project use a non-pathogen and not use animals. Our lab is of low risk and it has safety systems in place, for example eyewash bottle and fire extinguisher. The waste that is generated throughout our project will be disposed of in the biohazard bin. Our team's PI is an experienced biology teacher and is fully capable of overseeing student members and maintaining lab safety. Also, all student members in our team will only do experiments in the lab under the supervision of our team's PI and a qualified lab technician. Our team members have also received formal safety and security training.