ㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤ
February 2022
11/2
Brainstorm meeting
25/2
Brainstorm meeting
March 2022
21/3
Brainstorm meeting
30/3
Brainstorm meeting
May 2022
2/5
Brainstorm meeting
June 2022
17/6
Brainstorm meeting
Aug 2022
20/8
Recruit team member
September 2022
13/9
Confirmation of 2023 HKGTC iGEM topic
October 2022 - January 2023
Research proposal preparation
February 2023
3/2
Learning of Hill function
7/2
Data Science Experience Course-Plastic tagging activity
March 2023
20/3
First construct design
23/3
iGEM sharing session
April 2023
7/4
Beach-cleanup pre-trip
20/4
Interview with Dr. Wright
26/4
Data Science Experience Course-Plastic tagging activity
May 2023
10/5
iGEM sharing session
13/5
Designed the first prototype of Microplastics Data Collection Car
17/5
Interview with Leanne Tam from Greenpeace
20/5
Beach clean-up activity
23/5
AHL induction with construct #1 and #3
25/5
Seek advice from school teacher, Mr Alex O
Second design
27/5
Proposed 2nd and 3rd phase of machine making
30/5
AHL induction with construct #1 and #3
Interview with Prof. Patrick Lee from the City University of Hong Kong
31/5
Full proposal of machine made
June 2023
3/6
Proposed ‘making 3D graphs for microplastics distribution’ in phase 3
6/6
AHL induction with construct #1 and #3
7/6
Survey
11/6
Interview with Oxford University iGEM team
14/6
Survey, interview with Harvard University iGEM team
13/6
AHL induction with construct #1 and #3
14/6
AHL induction with construct #1 and #3
30/6
Rehearsal for green fashion show
July 2023
1/7
Starts to study the performance of biosensors
3/7
Green fashion show, interview with Prof. King Chow from The Hong Kong University of Science and Technology
4/7
Recruited Clifford Wong to assist the building and design of the machine
Interview with EPD
5/7
New design of the machine by Clifford
iGEM sharing session
6/7
Interview with Environmental Protection Department (EPD)
11/7
Collaboration meeting with Oxford
13/7
AHL induction with construct #1 and #4
14/7
Collaboration meeting with Harvard
15/7
Building the machine and making 3d printing models
19/7
AHL induction with construct #1, #3, and #4
20/7
AHL induction with construct #2, #1 2:1, and #1 3:1
21/7
AHL induction with construct #2, #1 1:1, #1 2:1, and #1 3:1
23/7
Meeting with Jonas Fok, advisor
28/7
AHL induction but with all negative samples
August 2023
1/8
AHL induction with construct #1, #2, #3, and #4 with addition of IPTG
2/8
AHL induction with construct #1 1:1, #1 2:1, and #1 3:1
AHL induction with construct #1 3:1
Collaboration meeting with Oxford
3/8
AHL induction with construct #1 1:1 and #1 3:1
September 2023
2/9
New design of AI car
11/9
Project exhibition
23/9
AHL induction with construct pSB1C3-LasR-pLasRL-EGFP
New design of AI car
Project logo design confirmed
26/9
AHL induction with 3 selected colonies of construct pSB1C3-LasR-pLasRL-EGFP, starting experiments with triplicate results
28/9
AHL induction with 1 selected colony of construct pSB1C3-LasR-pLasRL-EGFP with different types of AHL
30/9
AHL induction with 1 selected colony of construct pSB1C3-LasR-pLasRL-EGFP with AHL 3OC12-HSL and different AHL concentrations
October 2023
1/10
AHL induction with 1 selected colony of construct pSB1C3-LasR-pLasRL-EGFP with AHL 3OC12-HSL and different AHL concentrations
3/10
Project exhibition
5/10
Project exhibition
6/10
Project exhibition
7 - 12/10
Wiki content preparation