Notebook












QSMiD

Quorum Sensing system for Microplastics Detection

Content


ㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤ

February 2022

11/2

Brainstorm meeting

25/2

Brainstorm meeting

March 2022

21/3

Brainstorm meeting

30/3

Brainstorm meeting

May 2022

2/5

Brainstorm meeting

June 2022

17/6

Brainstorm meeting

Aug 2022

20/8

Recruit team member

September 2022

13/9

Confirmation of 2023 HKGTC iGEM topic

October 2022 - January 2023

Research proposal preparation

February 2023

3/2

Learning of Hill function

7/2

Data Science Experience Course-Plastic tagging activity

March 2023

20/3

First construct design

23/3

iGEM sharing session

April 2023

7/4

Beach-cleanup pre-trip

20/4

Interview with Dr. Wright

26/4

Data Science Experience Course-Plastic tagging activity

May 2023

10/5

iGEM sharing session

13/5

Designed the first prototype of Microplastics Data Collection Car

17/5

Interview with Leanne Tam from Greenpeace

20/5

Beach clean-up activity

23/5

AHL induction with construct #1 and #3

25/5

Seek advice from school teacher, Mr Alex O

Second design

27/5

Proposed 2nd and 3rd phase of machine making

30/5

AHL induction with construct #1 and #3

Interview with Prof. Patrick Lee from the City University of Hong Kong

31/5

Full proposal of machine made

June 2023

3/6

Proposed ‘making 3D graphs for microplastics distribution’ in phase 3

6/6

AHL induction with construct #1 and #3

7/6

Survey

11/6

Interview with Oxford University iGEM team

14/6

Survey, interview with Harvard University iGEM team

13/6

AHL induction with construct #1 and #3

14/6

AHL induction with construct #1 and #3

30/6

Rehearsal for green fashion show

July 2023

1/7

Starts to study the performance of biosensors

3/7

Green fashion show, interview with Prof. King Chow from The Hong Kong University of Science and Technology

4/7

Recruited Clifford Wong to assist the building and design of the machine

Interview with EPD

5/7

New design of the machine by Clifford

iGEM sharing session

6/7

Interview with Environmental Protection Department (EPD)

11/7

Collaboration meeting with Oxford

13/7

AHL induction with construct #1 and #4

14/7

Collaboration meeting with Harvard

15/7

Building the machine and making 3d printing models

19/7

AHL induction with construct #1, #3, and #4

20/7

AHL induction with construct #2, #1 2:1, and #1 3:1

21/7

AHL induction with construct #2, #1 1:1, #1 2:1, and #1 3:1

23/7

Meeting with Jonas Fok, advisor

28/7

AHL induction but with all negative samples

August 2023

1/8

AHL induction with construct #1, #2, #3, and #4 with addition of IPTG

2/8

AHL induction with construct #1 1:1, #1 2:1, and #1 3:1

AHL induction with construct #1 3:1

Collaboration meeting with Oxford

3/8

AHL induction with construct #1 1:1 and #1 3:1

September 2023

2/9

New design of AI car

11/9

Project exhibition

23/9

AHL induction with construct pSB1C3-LasR-pLasRL-EGFP

New design of AI car

Project logo design confirmed

26/9

AHL induction with 3 selected colonies of construct pSB1C3-LasR-pLasRL-EGFP, starting experiments with triplicate results

28/9

AHL induction with 1 selected colony of construct pSB1C3-LasR-pLasRL-EGFP with different types of AHL

30/9

AHL induction with 1 selected colony of construct pSB1C3-LasR-pLasRL-EGFP with AHL 3OC12-HSL and different AHL concentrations

October 2023

1/10

AHL induction with 1 selected colony of construct pSB1C3-LasR-pLasRL-EGFP with AHL 3OC12-HSL and different AHL concentrations

3/10

Project exhibition

5/10

Project exhibition

6/10

Project exhibition

7 - 12/10

Wiki content preparation