Overview:
We created 9 basic parts and 1 composite part.
BBa_K4946001
LasR sensing module
Basic part (Coding)
Length: 729bp
LasR is a coding sequence used for the construction of four whole-cell biosensors, pET21b-LasR-pUC57-pLasRL-EGFP, pET21b-LasR-pUC57-pLasR3-EGFP, pET23b-LasR-pUC57-pLasRL-EGFP, and pET23b-LasR-pUC57-pLasR3-EGFP with green fluorescence readout.
BBa_K4946002
LasRL inducible promoter
Basic part (Coding)
Length: 409bp
LasRL is an inducible promoter used for the construction of two whole-cell biosensors, pET21b-LasR-pUC57-pLasRL-EGFP and pET23b-LasR-pUC57-pLasRL with green fluorescence readout.
BBa_K4946003
LasR3 inducible promoter
Basic part (Coding)
Length: 397bp
LasR3 is an inducible promoter used for the construction of a whole-cell biosensor with green fluorescence readout.
BBa_K4946004
pSB1C3-LasR-pLasRL-EGFP
Composite part (Device)
Length: 2193bp
A combination of parts K4946001 and K4946002 which was integrated into a pSB1C3 plasmid.
BBa_K4946005
LasR-F1
Basic part (Primer)
Length: 20bp
This is the LasR F1 forward primer, for amplification of the LasR gene by PCR. The product size is 566bp.
BBa_K4946006
LasR-R566
Basic part (Primer)
Length: 20bp
This is the LasR R566 reverse primer, for amplification of the LasR gene by PCR. The product size is 566bp.
BBa_K4946007
pLasR3-F72
Basic part (Primer)
Length: 20bp
This is the pLasR3 F72 forward primer, for amplification of the LasR3 inducible promoter by PCR. The product size is 307bp.
BBa_K4946008
pLasR3-R378
Basic part (Primer)
Length: 21bp
This is the pLasR3 R378 reverse primer, for amplification of the LasR3 inducible promoter by PCR. The product size is 307bp.
BBa_K4946009
pLasRL-F27
Basic part (Primer)
Length: 18bp
This is the pLasRL F27 forward primer, for amplification of the LasRL inducible promoter by PCR. The product size is 274bp.
BBa_K4946010
pLasRL-R300
Basic part (Primer)
Length: 23bp
This is the pLasRL R300 reverse primer, for amplification of the LasRL inducible promoter by PCR. The product size is 274bp.
