Contributions
For our contributions, we created the following composite parts:
| Part ID | Part Name | Part Description |
|---|---|---|
| BBa_K4999000 | pBAD driven T4 lysis cassette | This part involves the T4 lysis gene that is induced by arabinose. This allows for controlled lysis of E. coli cells, which is useful in our application of cell-free ATPS systems. |
| BBa_K4999001 | LuxR driven by constitutive promoter | This part involves combining LuxR under a constitutive promoter, which creates the protein needed for quorum sensing. This part can be integrated with other Lux components to create a quorum sensing circuit. |
Candidate For Best Composite Part
Our significant contribution was designing a composite part (BBa_K4999000) that involved an arabinose inducible lysis system. Our composite part consists of the following basic parts:
- Arabinose inducible pBAD promoter (BBa_I0500)
- T4 Lysis coding sequence (BBa_K112808)
Plasmid Maps:
Gel Images:
Lysis Images:
DH5alpha E. coli Bacteria were transformed with the above plasmid. Lysis was shown for Clone #2 in the figure below.
Lysis Growth Curve:
As shown on the graph below, we have that the bacteria culture grown with arabinose lyse
Optical Density (OD) of Bacteria Culture (Proxy for Bacteria Concentration) over Time. The red curve as arabinose added in at the start, and the green curve has no arabinose added in