Overview
GEMS-Taiwan 2023 Team worked with 14 basical parts and 5 composite parts in total, contributing to 10 the registry this year.
The range of our parts number is BBa_K4852000 to BBa_K4852999. The following partss focus specifically on our project
rather than providing a general overview.
Basic Parts
|
Key:
🌊 = Existing Part 🐠 = New Part |
|---|
| Part Number | Part Name | Description | Type | Length |
|---|---|---|---|---|
| BBa_K3633015 🐠 | T7 Promoter | This is a strong constitutive promoter, which allows us to have high expression of csg. | Promoter | 19 bp |
| BBa_J23100🌊 | constitutive strong Anderson promoter(J23100) | Strong Promoter | Promoter | 35 bp |
| BBa_J23106🌊 | constitutive strong Anderson promoter(J23106) | Medium Strength Promoter | Promoter | 35 bp |
| BBa_K4852003🐠 | pCinI | The cinI promoter promotes the cinI gene sequence. | Promoter | 74 bp |
| BBa_K594009🐠 | praiI | It is a repressible promoter, repressed by PraR. CinS binds to PraR, and forms a 1:1 dead-end complex, leading to the increase in production of MetJ through AHL synthesis. | Promoter | 519 bp |
| BBa_B0034🌊 | RBS (Elowitz 1999) | Weak RBS | RBS | 12 bp |
| BBa_K4852006🐠 | PraR | PraR forms a 1:1 complex with CinS for MetJ production. | Coding | 417 bp |
| BBa_K594007🐠 | cinS | CinS forms 1:1 complex with PraR for MetJ production. | Coding | 204 bp |
| BBa_C0077🌊 | CinI/R | Part of a Quorum-Sensing Network | Coding | 1514 bp |
| BBa_K594007/ BBa_K594008🌊 | raiI/R | Part of a Quorum-Sensing Network Controlled by cinI and cinR. | Coding | 1365 bp |
| BBa_K4852002🐠 | MetJ | This is the coding sequence for the production of MetJ, a regulatory protein that halts mRNA transcription. When the repressor protein in binded to the operator, RNA polymerase is unable to bind to the operator. | Coding | 787 bp |
| BBa_K4852005🐠 | Met operator | MetJ binding site | Signaling | 19 bp |
| BBa_B0010🌊 | Terminator B0010 | Transcriptional terminator consisting of a 64 bp stem-loop. | Terminator | 80 bp |
| BBa_B0012🌊 | Terminator B0012 | Transcription terminator for the E. coli RNA polymerase. | Terminator | 41 bp |
| BBa_K4852043🐠 | CsgBAC | The csgB gene initiates the process of curli fiber assembly by acting as a nucleator for the polymerization of CsgA, the major curli subunit. Meanwhile, the CsgC protein acts as a chaperone that prevents the premature polymerization of CsgA. | coding | 1368 bp |
| BBa_K4852042🐠 | CsgEFG | This selection of csg genes from E. coli MG1655 was based on their known role in curli fiber expression, secretion, and biofilm. | Coding | 1641 bp |
| BBa_B0053🌊 | His Operon Terminator | This terminator is used as a downstream terminator in the pSB**3 series of plasmids. However, it's termination efficiency has never been characterized. But as it exists in each of the pSB**3 plasmids, it has a 2 bp deletion that occurred during it synthesis via primer annealing and extension. | Terminator | 58 bp |
| BBa_R0010🌊 | LacI Promoter | This part is an inverting regulator sensitive to LacI and CAP. It contains two protein binding sites. The first binds the CAP protein, which is generally present in E. coli and is associated with cell health and availability of glucose. The second binds LacI protein. | Promoter | 78 bp |
Composite Parts
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_K3973002 🌊 | This is our quorum sensing kill switch mechanism for our Biosafety construct. | Composite | 3547 bp |
| BBa_K4852045 🐠 | The Csg construct is responsible for the synthesis of biofilm and repression in the presence of the protein metJ. | Composite | 3130 bp |
| BBa_K4852048🐠 | The metJ construct regulates production of biofilm. | Composite | 5053 bp |