This contains all of our basic parts and biological devices constructed for our probiotic treatment. Basic parts are the simple pieces used to then create a biological device. Basic parts include basic promotors, terminators, RBS, and enzyme coding sequences. The biological devices are complete pieces composed of the basic parts. The biological devices directly translate into how our enzymes are produced within the probiotic solution. The following FSU parts have linkes below to the Parts Registry for more information.

Roseovarius sp. 217 TMM

Type: Basic Part

This is the basic part for our roseovarius species 217 TMM. This species of marine bacteria will, after converting TMA into TMAO, convert this into formaldehyde and ammonia. This is a basic part because it only contains the enzyme itself.

Human Flavin containing Dimethylaniline Monooxygenase 3

Type: Basic Part

The human FMO3 gene is the original gene within humans that produces the FMO3 enzyme, allowing our bodies to naturally oxidize TMA into TMAO. TMAU patients do not have this function, so we figured we investigate testing this enzyme itself in E. coli as well. This is a basic part because it only contains the enzyme itself.

R. pomeroyi TMM Generator

Type: Biological device

Ruegeria pomeroyi is a gram-negative, rod-shaped, aerobic coastal ocean bacteria. It was discovered discovered in 1974 by Lawrence "Larry" Pomeroy, a researcher at the University of Georgia. Ruegeria pomeroyi is from the Marine Roseobacter clade. Due to its ability to oxidize TMA at a possibly better rate than the human FMO3 enzyme, it was selected for testing. It is a biological device because it combines an anderson promoter for initiating transcription with the ribosome entry site that promotes initiation of protein synthesis with the enzyme itself and then a terminator to halt gene expression.

Roseovarius species 217 TMM Generator

Type: Biological device

Roseovarius species 217 is a gram-negative bacteria of the phylum pseudomonadota. It was isolated from surface seawater collected near Plymouth, England. Due to its ability to oxidize TMA and its stronger enzymatic performance, the enzyme from it was utilized in our project. It is a biological device because it combines an anderson promoter for initiating transcription with the ribosome entry site that promotes initiation of protein synthesis with the enzyme itself and then a terminator to halt gene expression.

Human FMO3 Generator

Type: Biological device

The Human FMO3 Generator is the corresponding biological device to the simple part listed above. It was chosen as a way to gain a comprehensive understanding of various types of enzymes, including the original one that is functional in the normal population. It is a biological device because it combines an anderson promoter for initiating transcription with the ribosome entry site that promotes initiation of protein synthesis with the enzyme itself and then a terminator to stop gene expression.

Ma, M. (2004). Taxonomy browser (Ruegeria Pomeroyi DSS-3). National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=246200

Subhraveti, P. (2020, October 25). Summary of Roseovarius sp. 217, version 27.1 Tier 3 Uncurated Database. Summary of roseovarius sp. 217, version 27.1. https://biocyc.org/organism-summary?object=GCF_000152845