Parts
Throughout the course of our project, we obtained the sequence, structure, and function of the Basic part through literature review. By constructing the composite part, we were able to apply it in experimental validation . Further details are as follows:
Basic Parts Table
| Name | Nickname | Type | Description | Length |
|---|---|---|---|---|
| BBa_K4766000 | SpyCatcher | coding | SpyCatcher can be attached to a protein so that it can capture proteins connected with SpyTag. | 288 bp |
| BBa_K4766001 | SpyTag | coding | SpyTag can be attached to a protein so that it can be captured by proteins connected with SpyCatcher. | 289 bp |
| BBa_K4766002 | SnoopCatcher | coding | SnoopCatcher is an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag. | 336bp |
| BBa_K4766003 | SnoopTag | coding | SnoopTag is an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopCatcher. | 336bp |
| BBa_K4766004 | DogCatcher | coding | DogCatcher is a protein that forms a spontaneous isopeptide bond with DogTag peptide | 312bp |
| BBa_K4766005 | DogTag | coding | DogTag is a protein that forms a spontaneous isopeptide bond with DogCatcher peptide | 69bp |
| BBa_K4766006 | Mi3 | coding | Mi3 is an engineered dodecahedral protein cage based on the aldolase of thermophilic bacteria,15 consisting of 60 copies of identical subunit with a diameter of 26 nm. | 615bp |
| BBa_K4766007 | mEGFP | coding | mEGFP is a basic (constitutively fluorescent) green fluorescent protein. Its excitation wavelength lies at 488nm and its emission wavelength at 507nm. | 717bp |
| BBa_K4766008 | SA | coding | The most common uses of streptavidin are the purification or detection of various biomolecules. The strong streptavidin-biotin interaction can be used to attach various biomolecules to one another or onto a solid support. Harsh conditions are needed to break the streptavidin-biotin interaction | 594bp |
Composite Parts Table
| Name | Nickname | Type | Description | Length |
|---|---|---|---|---|
| BBa_K4766009 | Mi3-SpyCatcher | coding | We linked SpyCatcher to Mi3 so that it can capture proteins connected with SpyTag. | 939bp |
| BBa_K4766010 | mEGFP-SpyTag | coding | Fluorescent protein mEGFP connected with SpyTag can be captured by proteins connected with SpyCatcher, playing a role as a signal molecule. | 801bp |
| BBa_K4766011 | SA-SpyTag | coding | Streptavidin connected with SpyTag can be captured by proteins connected with SpyCatcher. | 618bp |
| BBa_K4766012 | Mi3-SnoopCatcher | coding | We linked SnoopCatcher to Mi3 so that it can capture proteins connected with SnoopTag. | 978bp |
| BBa_K4766013 | Mi3-DogCatcher | coding | We linked DogCatcher to Mi3 so that it can capture proteins connected with DogTag. | 945bp |