Introduction:
We would like to provide a detailed explanation of our project timeline. In order to do so, we will regularly review and update our progress to ensure that we are on track to meet our project goals. By closely monitoring our timeline and progress, we can make any necessary adjustments and ensure that we deliver a high-quality final product on time and within budget.
Day 1:
-Team meeting and design logo, slogan
Day 2:
-Expert interviews
-Questionnaire survey
- Laboratory Safety Training
- Configuring LB media
- Autoclave, pour plate
- PCR amplification of GII.4-VP1, GII.17-VP1, and RV VP7 genes
- Inoculate with pGEX-4T-1 strain
Participation list:
Arron/Naomi/Hedy/Candy/Tom/John/Belinda/Leah/Fiona/Yoyo/Ben
Day 3:
-Expert interviews
-Product research
- Electrophoresis GII.4-VP1, GII.17-VP1 and RV VP7 gene
- Gel purification
- Plasmid extraction pGEX-4T-1
Participation list:
Arron/Naomi/Hedy/Candy/Tom/John/Belinda/Leah/Fiona/Yoyo/Ben
Day 4:
-Interview Summary
- Electrophoresis of plasmid pGEX-4T-1
- Gel extraction of the separated target DNA
- Homologous Recombination of pGEX-4T-1 and RV VP7
- Transformation-Heat shock of DH5 alpha into the plasmid
- Digestion of plasmid pGEX-4T-1
- Digestion of DNA VP1/VP7
- Ligation of pGEX-4T-1 and VP1
- PCR purification
Participation list: Arron/Naomi/Hedy/Candy/Tom/John
Day 5:
- Wiki Writing
- Survey questionnaire analysis
- PCR identification of transformants
- Pick monoclonal culture seed solution
- Plasmid extraction pGEX-GII.4-VP1, pGEX-GII.17-VP1, pGEX-VP7-GII.4-VP1 and pGEX-VP7-GII.17-VP1 were transformed into BL21(DE3) and Nissle 1917 cells - Spread plate screening
Participation list: Belinda/Leah/Fiona/Yoyo/Ben
Day 6:
- Business Plan
- Wiki Writing
- PCR for plasmid DNA
- Electrophoresis of plasmid DNA
- Extracting plasmid from E.coli
- Select monoclonal culture seed solution
- Sent plasmids for sequencing
Participation list: Arron/Naomi/Hedy/Candy/Tom/John
Day 7:
- Education Workshop
- Expand the volume of cultivated bacteria
- Electrophoresis
- IPTG protein induce
- DNA gel recovery
- Homologous recombination
Participation list: Belinda/Leah/Fiona/Yoyo/Ben
Day 8:
- Science Education
- Ultrasonication
- GST protein purification
- SDS-PAGE modified acrylamide color gel
Participation list: Belinda/Leah/Fiona/Yoyo/Ben
Day 9
- Pitch Financing Roadshow
- Enterprise Visit
- Denaturation of the protein
- Preparation of SDS-PAGE gel
- SDS-PAGE
- Dyeing and decolorization of protein gel
Participation list: Arron/Naomi/Hedy/Candy/Tom/John
Day 10
- Repeat construction procedure of plasmid pGEX-RV-GII.17-VP1
- Wiki documentation
Participation list: Belinda/Leah/Fiona/Yoyo/Ben
Day 11
- Pick monoclonal culture seed solution
- Plasmid extraction pGEX-VP7-GII.17-VP1 were transformed into BL21(DE3) and Nissle 1917 cells
- Spread plate screening
Participation list: Belinda/Leah/Fiona/Yoyo/Ben
Day 12
- Expand the volume of cultivated bacteria
- Electrophoresis
- IPTG protein induce
- DNA gel recovery
- Sent for sequencing
Participation list: Arron/Naomi/Hedy/Candy/Tom/John
Day13
- Preparation of SDS-PAGE gel
- SDS-PAGE
- Dyeing and decolorization of protein gel
Participation list: Arron/Naomi/Hedy/Candy/Tom/John
Day14
- IPTG protein induce under different conditions
- Wiki documentation
Participation list: Belinda/Leah/Fiona/Yoyo/Ben
Day 15
- Preparation of SDS-PAGE gel
- SDS-PAGE
- Dyeing and decolorization of protein gel
- Wiki documentation
Participation list: Belinda/Leah/Fiona/Yoyo/Ben
