Team:BNUZH-CHINA/PARTS

Overview
Since FeFighterS comes from a multi-system design, the parts we've built to support the project are quite diverse and novel. Our parts are mainly concentrated in the targeting module, the killing module and the safety module. We carefully submitted all the components we developed to perform one particular function into Basic Parts in Registry, and then each basic part was combined with others orderly to compose functional Composite Parts, and finally submitted in Registry.

Basic Parts

Name Short Description Long Description
BBa_K4737000 prpsM prpsM is the promoter of 30S ribosomal subunit protein S13 of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
BBa_K4737001 OmpA OmpA is the gene of outer membrane protein A of Escherichia coli str. K-12 substr. MG1655
BBa_K4737002 Anti-CEA scFv Anti-CEA scFv is the gene encodes anti-CEA scFv antibody from Mus musculus (GenBank: BAA19944.1)
BBa_K4737003 SopE The type III secretion system signal peptide from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
BBa_K4737004 GOx gox is the gene encodes Glucose oxidase (GOx) from Aspergillus niger
BBa_K4737005 SLC7A11-shRNA This part encodes an shRNA that can successfully knock down expression of SLC7A11
BBa_K4737006 GPX4-shRNA This part encodes an shRNA that can successfully knock down expression of GPX4
BBa_K4737007 pLtetO pLtetO is the promoter which can be repressed by tetR
BBa_K4737008 HlyA HlyA is the gene encodes Listeriolysin-O protein from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
BBa_K4737019 GFP-shRNA This part encodes an shRNA that can successfully knock down expression of GFP

Composite Parts

Name Short Description Long Description
BBa_K4737009 prpsM::GFP To express GFP constitutively
BBa_K4737010 pnirB::GFP To express GFP under the anaerobic environment
BBa_K4737011 prpsM::SopE-FLAG-GOx To express SopE-FLAG-GOx constitutively
BBa_K4737012 pnirB::HlyA-6×His To express HlyA-6×His under the anaerobic environment
BBa_K4737013 prpsM::Lpp-OmpA-scFv To express Lpp-OmpA-scFv constitutively
BBa_K4737014 pnirB::SopE-Flag-GOx To express SopE-FLAG-GOx under the anaerobic environment
BBa_K4737015 prpsM::T7 RNA polymerase-T7 promoter::SLC7A11-shRNA To express T7 RNA polymerase and SLC7A11-shRNA constitutively
BBa_K4737016 prpsM::T7 RNA polymerase-T7 promoter::GPX4-shRNA To express T7 RNA polymerase and GPX4-shRNA constitutively
BBa_K4737017 prpsM::tetR To express tetR constitutively
BBa_K4737018 pLtetO::LacI-T7 Promoter::Lac operate-Sok-trp promoter::trp operator-Mok/Hok To express LacI controlled by tetR, to express Sok controlled by LacI, and to express Mok protein and Hok protein constitutively
BBa_K4737020 prpsM::T7 RNA polymerase-T7 promoter::GFP-shRNA To express T7 RNA polymerase and GFP-shRNA constitutively
BBa_K4737021 prpsM::HlyA-6×His To express HlyA-6×His constitutively
BBa_K4737022 T7 promoter::Lac operator-GFP-pLtetO::LacI To express LacI controlled by tetR, to express GFP controlled by LacI
BBa_K4737023 prpsM::mRFP-pLtetO::LacI To express LacI controlled by tetR and to express mRFP constitutively
BBa_K4737024 tetR-pLtetO::GFP To express GFP controlled by tetR and to express tetR constitutively