Overview
Since FeFighterS comes from a multi-system design, the parts we've built to
support the project are quite diverse and novel. Our parts are mainly concentrated in the targeting
module, the killing module and the safety module. We carefully submitted all the components we developed
to perform one particular function into Basic Parts in Registry, and then each basic part was combined
with others orderly to compose functional Composite Parts, and finally submitted in Registry.
Basic Parts
Name | Short Description | Long Description |
---|---|---|
BBa_K4737000 | prpsM | prpsM is the promoter of 30S ribosomal subunit protein S13 of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
BBa_K4737001 | OmpA | OmpA is the gene of outer membrane protein A of Escherichia coli str. K-12 substr. MG1655 |
BBa_K4737002 | Anti-CEA scFv | Anti-CEA scFv is the gene encodes anti-CEA scFv antibody from Mus musculus (GenBank: BAA19944.1) |
BBa_K4737003 | SopE | The type III secretion system signal peptide from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
BBa_K4737004 | GOx | gox is the gene encodes Glucose oxidase (GOx) from Aspergillus niger |
BBa_K4737005 | SLC7A11-shRNA | This part encodes an shRNA that can successfully knock down expression of SLC7A11 |
BBa_K4737006 | GPX4-shRNA | This part encodes an shRNA that can successfully knock down expression of GPX4 |
BBa_K4737007 | pLtetO | pLtetO is the promoter which can be repressed by tetR |
BBa_K4737008 | HlyA | HlyA is the gene encodes Listeriolysin-O protein from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) |
BBa_K4737019 | GFP-shRNA | This part encodes an shRNA that can successfully knock down expression of GFP |
Composite Parts
Name | Short Description | Long Description |
---|---|---|
BBa_K4737009 | prpsM::GFP | To express GFP constitutively |
BBa_K4737010 | pnirB::GFP | To express GFP under the anaerobic environment |
BBa_K4737011 | prpsM::SopE-FLAG-GOx | To express SopE-FLAG-GOx constitutively |
BBa_K4737012 | pnirB::HlyA-6×His | To express HlyA-6×His under the anaerobic environment |
BBa_K4737013 | prpsM::Lpp-OmpA-scFv | To express Lpp-OmpA-scFv constitutively |
BBa_K4737014 | pnirB::SopE-Flag-GOx | To express SopE-FLAG-GOx under the anaerobic environment |
BBa_K4737015 | prpsM::T7 RNA polymerase-T7 promoter::SLC7A11-shRNA | To express T7 RNA polymerase and SLC7A11-shRNA constitutively |
BBa_K4737016 | prpsM::T7 RNA polymerase-T7 promoter::GPX4-shRNA | To express T7 RNA polymerase and GPX4-shRNA constitutively |
BBa_K4737017 | prpsM::tetR | To express tetR constitutively |
BBa_K4737018 | pLtetO::LacI-T7 Promoter::Lac operate-Sok-trp promoter::trp operator-Mok/Hok | To express LacI controlled by tetR, to express Sok controlled by LacI, and to express Mok protein and Hok protein constitutively |
BBa_K4737020 | prpsM::T7 RNA polymerase-T7 promoter::GFP-shRNA | To express T7 RNA polymerase and GFP-shRNA constitutively |
BBa_K4737021 | prpsM::HlyA-6×His | To express HlyA-6×His constitutively |
BBa_K4737022 | T7 promoter::Lac operator-GFP-pLtetO::LacI | To express LacI controlled by tetR, to express GFP controlled by LacI |
BBa_K4737023 | prpsM::mRFP-pLtetO::LacI | To express LacI controlled by tetR and to express mRFP constitutively |
BBa_K4737024 | tetR-pLtetO::GFP | To express GFP controlled by tetR and to express tetR constitutively |