We successfully demonstrated secretion with a novel secretion tag called the VNp (Vesicle Nucleating peptide). We worked with 34 parts in total, contributing 24 of them to the registry. Parts entered into the registry include the basic Biobrick parts for three distinct VNp variants, as well as composite parts that fuse phasin proteins to various secretion tags and reporters. Altogether, these parts focus on the secretion of PHB granules from E. coli. Parts are shown below.
BioBrick | Part Name | Description and Purpose | Status | Length (bp) |
---|---|---|---|---|
BBa_K4738000 | Human alpha-synuclein derived peptide VNp2 coding sequence | Basic, Secretion tag | NEW, previously in literature | 114 |
BBa_K4738001 | Human alpha-synuclein derived peptide VNp6 coding sequence | Basic, Secretion tag | NEW, previously in literature | 114 |
BBa_K4738002 | Human alpha-synuclein derived peptide VNp15 coding sequence | Basic, Secretion tag | NEW, previously in literature | 60 |
BBa_K2739004 | Phasin (PhaP) | Basic, Protein coding sequence | Existing | 582 |
BBa_Z0251 | T7 strong promoter | Basic, Constitutive promoter | Existing | 35 |
BBa_B0015 | B0010-B0012 T1-T7 double terminator | Basic, Terminator | Existing | 129 |
BBa_K128005 | 6xHis affinity tag | Basic, Affinity tag | Existing | 18 |
BBa_B0034 | RBS-B0034, RBS based on Elowitz repressilator | Basic, RBS | Existing | 12 |
pSB1A3 | pSB1A3 plasmid backbone | Basic, backbone | Existing | 2155 |
BBa_E0040 | Wild-type GFP from Aequeora victoria | Basic, coding, reporter | Existing | 720 |
BBa_K3114015 | Golden Gate mRFP Module Flipper | Composite, Device | Existing | 892 |
BBa_K1149051 | Hybrid Promoter PhaCAB | Composite, PHB Production Construct | Existing | 4271 |
BBa_K2549053 | Gly-Gly-Gly-Gly-Ser linker | Basic, Flexible Linker | Existing | 15 |
BBa_K4738010 | PhaP-His6 fusion protein with T7 promoter | Composite, Phasin secretion device | NEW | 776 |
BBa_K4738011 | His6-PhaP-HlyA fusion protein with T7 promoter | Composite, Phasin secretion device | NEW | 962 |
BBa_K4738012 | GeneIII-PhaP-His6 fusion protein with T7 promoter | Composite, Phasin secretion device | NEW | 830 |
BBa_K4738013 | TorA-PhaP-His6 fusion protein with T7 promoter | Composite, Phasin secretion device | NEW | 914 |
BBa_K4738014 | VNp2-PhaP-His6 fusion protein with T7 promoter | Composite, Phasin secretion device | NEW | 890 |
BBa_K4738015 | VNp6-PhaP-His6 fusion protein with T7 promoter | Composite, Phasin secretion device | NEW | 890 |
BBa_K4738016 | VNp15-PhaP-His6 fusion protein with T7 promoter | Composite, Phasin secretion device | NEW | 836 |
BBa_K4738020 | PhaP-GFP-His6 fusion protein with T7 promoter | Composite, Phasin-GFP fusion protein secretion device | NEW | 1511 |
BBa_K4738021 | His6-PhaP-GFP-HlyA fusion protein with T7 promoter | Composite, Phasin-GFP fusion protein secretion device | NEW | 1697 |
BBa_K4738022 | GeneIII-PhaP-GFP-His6 fusion protein with T7 promoter | Composite, Phasin-GFP fusion protein secretion device | NEW | 1565 |
BBa_K4738023 | TorA-PhaP-GFP-His6 fusion protein with T7 promoter | Composite, Phasin-GFP fusion protein secretion device | NEW | 1649 |
BBa_K4738024 | VNp2-PhaP-GFP-His6 fusion protein with T7 promoter | Composite, Phasin-GFP fusion protein secretion device | NEW | 1625 |
BBa_K4738025 | VNp6-PhaP-GFP-His6 fusion protein with T7 promoter | Composite, Phasin-GFP fusion protein secretion device | NEW | 1625 |
BBa_K4738026 | VNp15-PhaP-GFP-His6 fusion protein with T7 promoter | Composite, Phasin-GFP fusion protein secretion device | NEW | 1571 |
BBa_K4738030 | Hybrid PhaCAB composite w/PhaP-GFP-His6 and T7 promoter | Composite, PHB production and secretion device | NEW | 5782 |
BBa_K4738031 | Hybrid PhaCAB composite w/His6-PhaP-GFP-HlyA and T7 promoter | Composite, PHB production and secretion device | NEW | 5968 |
BBa_K4738032 | Hybrid PhaCAB w/GeneIII-PhaP-GFP-His6 and T7 promoter | Composite, PHB production and secretion device | NEW | 5836 |
BBa_K4738033 | Hybrid PhaCAB composite w/TorA-PhaP-GFP-His6 and T7 promoter | Composite, PHB production and secretion device | NEW | 5920 |
BBa_K4738034 | Hybrid PhaCAB composite w/VNp2-PhaP-GFP-His6 and T7 promoter | Composite, PHB production and secretion device | NEW | 5896 |
BBa_K4738035 | Hybrid PhaCAB composite w/VNp6-PhaP-GFP-His6 and T7 promoter | Composite, PHB production and secretion device | NEW | 5896 |
BBa_K4738036 | Hybrid PhaCAB composite w/VNp15-PhaP-GFP-His6 and T7 promoter | Composite, PHB production and secretion device | NEW | 5842 |