This page contains the contributions we made towards existing parts (Chart 1). We hope that the characterization we made and our experience of using these parts can benefit future teams.

Cas9 and Lambda-Red

For Cas9 (BBa_K1218011) and Lambda-Red (BBa_K1433005), combining them, we conducted characterizations involving the editing efficiency of the genome and plasmids. What's more, we shared some technical insights learned from our experiments, which may serve as references for others.

EL222 system and pEL222

For the EL222 system (BBa_K2447501), we identified a missing fragment in the part's sequence. We registered a new part (BBa_K4630301) to rectify this error and conducted our own characterization. Furthermore, we performed directed evolution on pEL222 (EL binding site, BBa_M45400) in three directions: reducing leakage and raising/lowering, with details available in the new part we registered (BBa_K4630300).

Thiosulfate sensing system, Pluxr, pPcau

For the parts in “Applications”, as we didn't receive the synthesized sequences before the WiKi freeze day, we supplemented these parts with new information gathered from literature sources. We added the characterization of literature to the thiosulfate sensing system (BBa_K3733032), Pluxr (BBa_R0062), and pPcau (BBa_K2825002).