What are nanoparticles?
In recent times, nanoparticles containing metallic elements, particularly Iron Oxide Nanoparticles (IONPs), have gained significant prominence. IONPs are lauded for their exceptional biological compatibility and inherent ferromagnetism.
This is why our program is dedicated to the study of IONPs, as they offer a diverse range of applications. For instance, in the realm of cancer therapy, IONPs can generate heat that selectively targets and eradicates tumor cells.
Our genetically edited E. coli is used to synthesize Iron Oxide Nanoparticles (IONPs) by adding Fe3+ for induction.
After leaving the system in shaker for 1-2 days, we can seperate the IONPs from the culture by untrasonification and filtration method designed and tested by us own.
What is special about our nanoparticles?
They are not just any other nanoparticles! They have special abilities due to their properties. We then step into exploring therapeutic applications and drug delivery possibilities.
We focused on breast cancer and used a pGLO plasmid that had previously demonstrated protein expression with visible green-fluorescent protein (GFP). To target HER2, we replaced the original protein-coding gene with the anti-HER2 scFv coding gene. This modified gene includes a 6x His-tag for protein detection and purification.
We designed a method for the separation of the Iron Oxide Nanoparticles by taking the advantage of ultrasonication and ultrafiltration. This method could relatively precisely separate the nanoparticles of desired size from the biological masses, resulting in a satisfying low polydispersity index, which is also an important factor to control for application. What is especially important is that the method designed by us is nicely suitable for preliminary test, as this method largely reduced the interference factors like salt ions and aggregation in the characterization like TEM and DLS.
Our experiments range from plasmid design, bacterial culture, to protein purification, chemistry to cell experiments. All of our experimental procedures and operations are documented in detail here.
1. We successfully synthesized the Iron Oxide Nanoparticles (IONPs) E. coli and the method used to separate
IONPs are designed and tested by ourselves.
2. We successfully characterized the IONPs using TEM and DLS and found that using ok our method, the IONPs
got by us were generally in superparamagnetic state and suitable for application need magnetic induction and
won't let particles aggregrate.