RESULTS

1. Single mutant (S491A) mhetase transformant in E. coli BL21 DE3

2. Double mutant (S196A, T434S) mhetase transformant in E. coli BL21 DE3


Fastpetase transformant in E. coli BL21 DE3



Mhetase transformant in E. coli BL21 DE3

LEGEND
NTC – No template control (Negative control)
PP – Plasmid (Positive Control)
MM – PCR Master mix

qPCR quantification data shows the abundance of FAST-PETase in E. coli DH5α

qPCR quantification data shows the abundant expression of FAST-PETase in E. coli BL21 (DE3)

qPCR quantification data shows the abundance of wild type MHETase in E. coli DH5α

PCR quantification data shows the abundant expression of Wild type MHETase in E. coli BL21 (DE3)