RESULTS
- Single mutant (S491A) mhetase transformant in E. coli BL21 DE3
- Double mutant (S196A, T434S) mhetase transformant in E. coli BL21 DE3
![](https://static.igem.wiki/teams/4625/wiki/image-20.png)
![](https://static.igem.wiki/teams/4625/wiki/image-23.png)
Fastpetase transformant in E. coli BL21 DE3
![](https://static.igem.wiki/teams/4625/wiki/image-24.png)
Mhetase transformant in E. coli BL21 DE3
LEGEND
NTC – No template control (Negative control)
PP – Plasmid (Positive Control)
MM – PCR Master mix
![](https://static.igem.wiki/teams/4625/wiki/wetlab1.png)
qPCR quantification data shows the abundance of FAST-PETase in E. coli DH5α
![](https://static.igem.wiki/teams/4625/wiki/wetlab2.png)
qPCR quantification data shows the abundant expression of FAST-PETase in E. coli BL21 (DE3)
![](https://static.igem.wiki/teams/4625/wiki/wetlab3.png)
qPCR quantification data shows the abundance of wild type MHETase in E. coli DH5α
![](https://static.igem.wiki/teams/4625/wiki/wetlab4.png)
PCR quantification data shows the abundant expression of Wild type MHETase in E. coli BL21 (DE3)