Broke a centrifuge, broke an agarose gel 4 consecutive times, attempted the same clonage for 8 times
Francesca Galiazzo
Biotechnology
Fan and lover of sepiolite, among other unsuccessful lab tasks…
Marco Costanzo
Medicine & Surgery
Prepared 2 PCR reactions to run simultaneously ignoring that the second one needs the amplified fragment of the first one
Niccolò Venturini Degli Esposti
Biomedical Engineering
I made an agarose gel with milliQ water instead of TAE buffer, and ran it.
Chiara Zanin
Biotechnology
I used dry ice for bacteria chemical transformation, dropped an entire plate reader before loading it (96-well).
Luca Guarnieri
Biomedical Engineering
I diluted 100ul LB in 900ul LB and plated on an LB petri plate… surprisingly nothing grew, but sterility was achieved
Asia Picchi
Biology
I made bacteria explode while electroporating. Spoiler: somehow it worked.
Chiara Ravazzolo
Biotechnology
I forgot to add agarose in gel and wait more than half an hour for it to solidify… spoiler: it has never.
Alex Martini
Biotechnology
I opened a plate with a week's worth of sterile work just because I wanted the condensation to dry.
Valentina Del Giudice
Biotechnology
I love to do the growth curves and leave the lab at 10 pm.
Giorgia D'Angelo
Biotechnology
Prepared LB broth by mixing every single ingredient for months before discovering a ready-to-use mix.
Sara Letrari, MSc
Instructor
PhD Student at the Department of Molecular Medicine
Livio Trainotti
Primary P.I.
Associate professor of General Botanics at the Department of biology
Massimo Bellato
Secondary P.I.
Post-Doc researcher at the Department of Information Engineering
Claudia Del Vecchio
Advisor
Associate Professor of Microbiology at the Department of Molecular Medicine
Cristiano De Pittà
Advisor
Associate Professor of Genetics at the Department of Biology
Let us introduce you to the bacteria we worked with in the same way we used to refer to as they were human beings (Disclaimer: this in not meant to be offensive in any way but it’s just for fun!).
E. coli
(Eschi) is a university student who during the daytime attends lessons and lab activities but in the evening loves hanging out and having a drink with his girlfriend of the month. His favorite musical genre is 1990s rock.
P. aeruginosa
(Pseudo) is a radical chic woman in her forties who has a season ticket to the opera house and during weekends holds cultural meetings with her friends. Her favorite musical genre is classical music.
K. pneumoniae
(Klebby) is a stubborn elderly man who loves spending time watching people working in construction sites and playing card games with his lifelong friends. His favorite musical genre is 1950s classic pop.
A. baumannii
(Aci) is a rebellious high school girl who is inseparable from her friends with whom she sometimes skips classes and pretends to be older to go clubbing on Saturday nights. Her favorite musical genre is techno music.