1.To enter the laboratory, you must wear the necessary work clothes according to the regulations;

2.Do not wear contact lenses during the experiment;

3.When operating high-temperature experiments, you must wear anti-high-temperature gloves to avoid burns;

4.Do not eat or drink food in the laboratory;

5.When handling hazardous chemicals, please be sure to comply with the operating code follow the teacher's operating procedures, or conduct experiments

6.The laboratory must be kept clean and tidy every day;

7.Electrical sockets do not connect too many plugs, so as not to charge the load, causing an electrical fire;

8.The container can be used only when the purpose of the container is confirmed

9.Toxic and irritating waste should be collected and disposed of in a special trash can

10.Be careful about the cutting edge when using sharp types of equipment like knives

1.We only use E. coli in our experiments for genetic engineering, including DH5-alpha and BL21 and they were purchased from Sangon Biotech company.

2.We ordered our DNA materials from Genscript company and the plasmid backbone, pET28a vector, was provided by SubCat.

1.All members of the wet team received safety training before we started our experiments.

2.All lab work was conducted under the supervision of our instructor and the lab manager