*Last update of this notebook happened on 2023/08/10 and the detailed protocol can be found on the Experiment page.

0729

Team online meeting: experiment design and confirm intensive laboratory schedule.

0729

Team offline meeting: team logo design and teamwork allocation.

Order primers by the instructor.

0730

Laboratory safety training and basic instrument training.

Preparation of LB medium and E. coli cultivation.

0731

PCR for target genes and recycle.

Plasmid extraction for pET28a vector.

0801

Double enzyme digestion, plasmid extraction and ligation.

PCR and electrophoresis gel for validation.

0802

Repeat Double enzyme digestion, plasmid extraction and ligation.

BL21 transformation.

0803

PCR and electrophoresis gel for validation.

LB medium preparation and E. coli cultivation.

0804

OD detection and IPTG induced expression.

0805

Coomassie brilliant blue dye and run SDS-PAGE gel.

0806

Repeat Coomassie brilliant blue dye and run SDS-PAGE gel.

0807

Break day.

0808

Function test---Fret by the microplate reader.

0809

Fret Results analysis.

0810

Sequencing result analysis.