This project has a biosafety level of 2. To manage the amount of risk in this project, a strict set of rules were followed and written procedures were planned and approved by the Chulalongkorn lab advisors to ensure proper biosafety. Proper training on decontamination, biological waste management, personal protective equipment, safe handling of biological spills, and the transportation of biological materials were received from the Chulalongkorn lab advisors. This allowed the team to upload lab rules to a high standard. Additionally, the team was provided with the proper personal protective equipment, biosafety supplies, and training for this project. ^[1-7]

    For example, when working with A. tumefaciens, a biosafety cabinet was used to prevent the potential effects of inhalation or skin contact. When agroinfiltrating N. benthamiana, safety glasses and lab coats were used to prevent the A. tumefaciens from entering the eyes.

    Thailand-RIS team members did not take any substances outside of the lab and did not bring any inside (i.e. food). The lab used is one that is used by many other Chulalongkorn biologists who, alongside the Thailand-RIS team, adhere to strict regulations^[1-7] to remain safe for biosafety level 2 experiments. Materials were autoclaved as needed in order to avoid contamination and spread. Gloves and other equipment were properly disposed of into a designated trash bin for biological waste.

Agroinfiltration of N. benthamiana with gloves, lab coats, and safety goggles. Safety goggles were used during agroinfiltration to prevent the solution from entering the eyes. Biosafety cabinet used when creating the agroinfiltration solution.

References

1. Chuanchuen R. Biological Spills - Response and Clean Up. Cuvetamr.vet.chula.ac.th. September 16, 2020. Accessed June 13, 2023. http://www.cuvetamr.vet.chula.ac.th/media/content/file/2021/04/CONTENT_FILE_35_20210421083831.pdf.
2. Chuanchuen R. Hands on Practice - PPE. Cuvetamr.vet.chula.ac.th. September 16, 2020. Accessed June 13, 2023. http://www.cuvetamr.vet.chula.ac.th/media/content/file/2021/04/CONTENT_FILE_35_20210421082952.pdf.
3. Chuanchuen R. Decontamination. Cuvetamr.vet.chula.ac.th. September 16, 2020. Accessed June 13, 2023. http://www.cuvetamr.vet.chula.ac.th/media/content/file/2021/04/CONTENT_FILE_35_20210421082830.pdf.
4. Biosafety Equipment — PPE and BSCs. Cuvetamr.vet.chula.ac.th. Accessed June 13, 2023. http://www.cuvetamr.vet.chula.ac.th/media/content/file/2021/04/CONTENT_FILE_35_Biosafety%20equipment_PPE%20BSC.pdf.
5. Biosafety Equipment - Decontamination & Disinfection. Cuvetamr.vet.chula.ac.th. Accessed June 13, 2023. http://www.cuvetamr.vet.chula.ac.th/media/content/file/2021/04/CONTENT_FILE_35_20210421075056.pdf.
6. Chuanchuen R. Biological Waste Management. Cuvetamr.vet.chula.ac.th. September 16, 2020. Accessed June 13, 2023. http://www.cuvetamr.vet.chula.ac.th/media/content/file/2021/04/CONTENT_FILE_35_20210421075030.
7. Puangseree J. Transportation of Biological materials. Cuvetamr.vet.chula.ac.th. September 16, 2020. Accessed June 13, 2023. http://www.cuvetamr.vet.chula.ac.th/media/content/file/2021/04/CONTENT_FILE_35_20210421083845.pdf.