All our work is covered by the White List.
For the development of the biosensor, we used EryK, EPCP, mVENUS and AtPCS as constituent parts which are non-hazardous.
Additionally we used two E. coli strains that don't pose a threat to human health or safety as they are not pathogenic in nature : TOP10 as a maintenance strain and BL21 as an expression strain. Nevertheless, at the end of working with modified strains, they are either stored in a designated space at 4 ºC or -80 ºC or sterilized with a 3% chlorine solution and discarded in a designated waste bag This is all in accordance to the guidelines generated by the Mexican Accreditation Entity (EMA) and the Mexican Biosecurity Association (AMEXBIO) to avoid releasing any genetically engineered organisms into the environment.
In order to conduct experiments in the laboratory, it is necessary to enter with a lab coat, long pants that cover the legs, closed shoes, gloves, and, if needed, safety glasses. We prioritize working in pairs whenever possible and avoid working alone, except in situations that necessitate it in which case a supervisor or an advisor is always aware of our whereabouts in the lab. All members have completed a safety course, and are always accompanied by a more experienced lab member when carrying out experiments. Furthermore, we have a safety protocol in place, complete with reagent warnings, emergency and evacuation protocols, and proper disposal instructions to ensure the well-being of all participants. This safety approach not only ensures the safety and reproducibility of our work but also drives progress and will allow for a seamless transition to the next generation of our team.
All team members attended a laboratory safety course before we began with laboratory experiments. The following topics were reviewed: proper laboratory practices, access to emergency exits, chemical safety, biosafety guidelines, sterilization techniques, waste disposal, location of first aid kit, location of eyewash and shower, and emergency procedures. Additionally, campus safety services have protocols in place in case of earthquakes and fires.