Date: 08/04/2023
Event: 1st
online theory training for whole members
Agenda:
iGEM
competition background information training
Date: 15/04/2023
Event: 2nd
online theory training for wet lab
Agenda:
Academic
frontier of genetic engineering - Literature reading and analysis of classical routes of genetic
engineering.
Date: 22/04/2023
Event: 3rd
online theory training for dry lab
Agenda:
How
to tap into market demand - questionnaire design and market research methods.
Date: 29/04/2023
Event: 4th
online theory training for wet lab
Agenda:
Essential
for Introduction to Biological Experiments - Basic Research Methods of Modern Molecular Biology 1.
Date: 06/05/2023
Event: 5th
online theory training for dry lab
Agenda:
Hands-on
teaching you to complete a high-quality interview - expert interview tips and examples.
Date: 13/05/2023
Event: 6th
online theory training for wet lab
Agenda:
Advanced
Teaching of Biological Experiments-Basic Research Methods of Modern Molecular Biology 2.
Date: 20/05/2023
Event: 7th
online theory training for dry lab
Agenda:
How
to creatively disseminate scientific information - popular science education activities and
materials.
Date: 27/05/2023
Event: 8th
online theory training for whole members
Agenda:
Use
synthetic biology to "build blocks" - biological elements and gene circuits.
Date: 03/06/2023
Event: 9th
online theory training for whole members.
Agenda:
From
0 to 1: The Road to Product Commercialization - Business Planning and Product Implementation
Strategy
Date: 10/06/2023
Event: 10th
online theory training for whole members
Agenda: "Speak" with data-modeling methods and diagrams to teach.
Date: 17/06/2023
Event: 11th
online theory training for whole members
Agenda: Demystifying The Rule Off iGEM Challenge I
Date: 24/06/2023
Event: 12th
online theory training for whole members
Agenda: Demystifying The Rule Off iGEM Challenge Ⅱ
Date: 20/07/2023
Event: offline training with both wet
and dry lab
Agenda: Self-introduction, reserve captain campaign, background explanation of the topic. Team discussion: team name, logo, Slogan, VI, Team, Mission, uniform, social platform account.
Date: 21/07/2023
Event: offline training with
separated wet and dry lab
Agenda: 1. Learn the safety rules.
2. Configure LB solution.
3. Complete the process of PCR system.
4. Prepare target gene fragment CBD and MS18 solution.
5. Prepare target plasmid PET28A samples.
Date: 22/07/2023
Event: offline training with
separated wet and dry lab
Agenda: 1. Extract empty target plasmid.
2. Add restriction enzymes.
3. Finish the process of homologous recombination.
4. Extract target DNA gel.
5. Transform recombinant products.
6. Coat the plate with bacteria.
Date: 23/07/2023
Event: offline training with
separated wet and dry lab
Agenda: 1. Pick monoclonal bacteria and culture it by shaking.
2. Finish the process of PCR of bacteria solution.
3. Configure stock liquid of Tris Buffer, NaCl solution, imidazole solution.
4. Configure SDS-page gel.
Date: 24/07/2023
Event: offline training with
separated wet and dry lab
Agenda: 1. Configure LB solution.
2. Shake bacteria solution.
3. Configure SDS-page gel.
4. Configure Tris-HCl.
5. Configure His Buffer A and His Buffer B.
6. Measure absorbance of samples by Microplate Spectrophotometer
7. Extract the plasmid and convert the validated recombinant plasmid into BL21.
Date: 25/07/2023
Event: offline training with
separated wet and dry lab
Agenda: 1. Expand cultivation
2. IPTG induced protein expression（Overnight）
Date: 26/07/2023
Event: offline training with
separated wet and dry lab
Agenda: 1. Extract the target protein by high-speed centrifugation and ultrasonic crushing;
2. protein purification by nickel column (6x His).
3. Dialysis (overnight)
Date: 27/07/2023
Event: offline training with
separated wet and dry lab
Agenda: 1. Determination of protein concentration;
2. SDS PAGE analysis;
3. designing experiments to map out the optimal reaction conditions of the system: different concentrations of TMB and different enzyme-catalyzed reaction times (model)
Date: 28/07/2023
Event: offline training with
separated wet and dry lab
Agenda: 1. System function test: detect different concentration gradients of bacterial solutions
2. draw standard curves through the results, as the basic data of the app for detection.
Date: 30/07/2023
Event: offline training with both wet
and dry lab
Agenda: 1. Write lab reports and related text materials.
2.Prepare for the internal presentation rehearsal.
Date: 31/07/2023
Event: offline training with both wet
and dry lab
Agenda: Internal presentation rehearsal
Competition Milestones
Date: 14/09/2023
Event: Team Project Promotion Video Submission
Date: 18/09/2023
Event: Preliminary Judging Form Submission
Date: 12/10/2023
Event: 1. Wiki Freeze.
2. Judging Form Freeze.
3. Project Attributions.
4. Form Freeze.
5. Registry Part Pages Freeze.
6. Project Title and Abstract.
Date: 19/10/2023
Event: 1. Presentation Video Submission
Date: 2~5/11/2023
Event: Grand Jamboree
Date: 16/11/2023
Event: Wiki Thaw.
Date: 14/12/2023
Event: 1. Wiki Archive.
2. 2023 Competition Closing