-PREPARE
l Read the experimental notes and know what you will be doing today.
l Make sure you understand all purposes and processes about the experiment.
l Assign tasks to everyone in the team.
-IN THE LAB
l When experimenters do some experiments or touch equipments of experiments in the lab, they should wear disposable lab gloves, lab coats and disposable mask.
l Lab coats and gloves MUST NOT be worn out of the laboratory.
l When using every equipments, experimenters should know operational procedures and considerations.
l Don’t operate lab equipments without permission.
l Don’t eat or drink in the lab.
l Don’t taste any solution and substances in the lab.
-AFTER EXPERIMENTS
l Wash hands when you finish experiments.
l Clean up equipments and lab bench.
l All disposable equipment or used bacteria will be collected centrally.
- OPERATION OF THE PIPETTE
l Work on the lab bench.
l Don’t touch the pipette tip by hand.
l When there are any liquid in the pipette tip, hold the pipette vertically.
l When using the pipette to aspirate the liquid, it should not be sucked quickly, which will cause the solution to be sucked into the pipette.
l In order to reduce the splashing of liquid, the liquid is transferred into the container, and the tip is entered into the solution (but according to the experimental requirements)
l The tip must be replaced each time the liquid is removed.
l After using the pipette, adjust the pipette to the maximum range.
- OPERATION OF THE CENTRIFUGE
l The centrifuge should be placed and used horizontally.
l Before using the centrifuge, place the sample in equilibrium in the centrifuge, and if it is only a single tube, the other tube should take an equal mass of water instead.
l The lid of the centrifuge should be closed tightly.
- OPERATION OF THE PCR MACHINE
l When using the PCR machine, the environment should be constant.
l The power supply used by the thermal cycler should be stable.
l There should be no cross-contamination between samples.
- OPERATION OF THE SHAKE MACHINE
l We shake the bacteria on a shaker because the container is airtight, which ensures that the fungi are not infected with each other.
l We make sure the temperature is constant.
Our team used two competent states of E. coli：BL21 & DH5a
l We do each step of transferring microorganisms or genes, lighting alcohol lamps and alcohol disinfection of instruments, all in a sterile environment.
l These bacteria will be a little harmful to our health, so we will disinfect them before use.
Our experiments do not contain any human or animal parts or genes. All genetic modifications established in our process are for research purposes only.