1. Do not enter the lab without permission

2. Make sure your working area is safe

3. Make sure that electrical equipment is safe and well-prepared

4. No food or drink in laboratory

5. No running or horseplay in laboratory

6. Lab gloves and lab coats are required

7. No lighting a fire without instructions

8. Never leave an unattended Bunsen burner on a blue flame

9. No touching unrelated chemicals or reagents

10. Do not taste or sniff chemicals

11. Litter waste into specific bins

12. No taking any lab equipment away from laboratory

13. Unrelated people are prohibited entering laboratory

14. Know emergency exit routes

15. Minimize all chemicals exposures

16. Label the reagents properly

17. Long hair and lose clothing must be pulled back and secured from entanglement or potential capture

18. Wash exposed areas of the skin prior to leaving the laboratory

19. Never leave containers of chemicals open

20. Use equipment only for its designated purpose

21. Combine reagents in appropriate order

22. Concentrate on your lab work

 

1. PCR

· Clean and disinfect the laboratory according to the requirements before and after the inspection, especially after the completion of the experiment, the disinfection work must be thorough and destroy the residual DNA.

· Reduce the frequency of PCR experiments.

· During the PCR purchase procedure, staff must wear gloves and change them frequently.

· High temperature disinfection of pipettes, Rppendor tubes, reaction tubes, sample dosers, centrifuge tubes and other items should be done before each purchase.

· Conventional consumables are treated once after use to avoid pollution caused by repeated use.

· Instantaneous centrifugation before the reagent tube is used to make the liquid sink to the bottom of the tube and reduce the chance of contaminating gloves and dispenser.

· Correct use of the sampler.

 

2. Centrifuge

· Corrosion in the rotor cavities or exterior surfaces

· Scratches or gouges to the base metal

· Missing or worn anodising

· Damage to contact points, such as thread, hubs, and screws

 

3. Ultrasonic Cell Disruptor

· Ultrasonic frequency, intensity, and duration affect algal cell disruption.

· Acoustic cavitation, heat, pressure and free radicals are the major mechanisms.

· Hybrid techniques of ultrasound and other disruption methods reduce energy cost.

· Target product release is a vital indicator to reflect cell disruption degree.

· Research on system design and quality control for commercial use are needed.

 

4. Gel Electrophoresis

Potential hazards:

· Ethidium bromide – mutagen, irritant

· Acrylamide – carcinogen, neurotoxin, irritant

· Phenol – corrosive, toxic

· Chloroform – suspect carcinogen, toxic

How to use the equipment:

· Don’t run equipment unattended.

· Keep equipment clear of unintentional grounding points and conductors.

· Gel chamber must have a lid or cover with safety interlocks to prevent accidental contact with energized electrodes or buffer solutions.

· Gel chamber exterior must be dry with no spilled solutions. Check the chamber for leaks.

· Switch off all power supplies and unplug the leads before opening the gel chamber lid or reaching inside the gel chamber. Do not rely on safety interlocks.

 

5. Alcohol burner

· Typical fuel is denatured alcohol, methanol, or isopropanol.

· A cap is used as a snuffer for extinguishing the flame.

 

6. Clean Bench

· Airflow monitors to prove velocities

· Leak testing ports are in place

· No lights are present inside the hood

· No framing is used on directional flow shield/sash

· Particle counters are movable & used near the point of criticality

· A robust testing procedure is designed & performed repeatedly with video taping

· Have a removable perforated screed below the Fan power HEPA unit to produce better unidirectional flow

· Use a stainless-steel work surface pulled off the back wall to allow flow to keep the rear/sides of the table & wall clean. Must be movable.

 

7. E-Gel Imager

· Verify that all devices are turned off before making any connections.

· Do not block the ventilation openings of any parts of the E‑Gel™ Imager unit or of its sub-assemblies.

· Place the E‑Gel™ Imager unit at least 30 cm (12 inches) away from the walls and ceiling.

· Do not store below −10°C. The recommended operating conditions for the E‑Gel™ Imager are 25°C (78°F) ± 5°C, 55% relative humidity, up to 2000 m altitude. Storage conditions: 10°C to 40°C; 50–80% humidity; up to 2000 m altitude.

· Do not store the system in direct sunlight or in the direct flow of the air conditioner.

· Do not clean the system with harmful solvents. Only use a soft cloth dampened with water.

· Intended for indoor use only

 

 

8. Water Bath

· Wear safety goggles when placing or removing items from the water bath

· Do not leave unattended for long periods of time – the user must allow for loss of water through evaporation and must ensure that it does not evaporate to dryness.

· Take care when raising and removing the lid, it may be hot. Steam and hot vapours can scald.

· Ensure that the vessel and the liquid it contains is compatible with the chosen operating temperature.

· Do not block or restrict ventilation slots.

· Disconnect the unit from the power supply before filling or emptying the water bath.

· Do not top-up or empty the water bath until the water has cooled to room temperature.

· If the ALARM lamp is illuminated do not touch the liquid or the heater. Contact the Responsible Person or Laboratory Manager.

 

 

1. Microorganisms:

· Bacteria: E.coli DH5-alpha(non-pathogenic strain) , ordered from company 

· Yeast cell: Yeast 1974 (harmless to human body), gift from WANG XIN

· GA plasmid: provided by SubCat.

· Other DNA fragments: ordered from company

2. All enzymes and genetic materials are harmless or have very low risk to human body

· Amylase

· Glucoamylase

· X-2, XII-5, XI-2, X-3 plasmids

· CCTCC M94055, GA, temA, pHCas9

3. Agarose and TAE buffer for gel preparation are harmless

4. 2mix and Cut Smart Buffer for PCR system preparation are processed before usage

 

Our research and experiment didn’t refer to any human or animal part or gene. Anatomy of animal or human tissues is not involved in our experiment at all. What’s more, our experiment didn’t do harm to the environment, and all reagents are strictly prohibited being brought outside the lab. Waste is also littered in the specific bin to avoid further pollution.