Short Description:
Overexpression dxs, ispA, idi under the control of T7 promoter
Long description:
This plasmid is used to enhance the production of isoprenoid precursor in E.coli by overexpression three key enzyme in the MEP pathway under the control of T7 promoter, the three genes are 1-deoxyxylulose-5-phosphate synthase (DXS), farnesyl diphosphatesynthase (IspA) and isopentenyl diphosphate isomerase (IDI) from E.coli.
Short Description:
Introduce new pathway to produce isoprenoid in Ecoli
Long description:
This part comprise three genes, including SccK,AtiPK,idi.They work together to consecutively catalyze prenol and isoprenol to form DMAPP and IPP, which are the precursor of isoprenoid.