This team use yeast as a host for de novo synthesis of terprenoids. All terpenoids are built from C5 blocks: isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). IPP and DMAPP are naturally produced either through the mevalonate (MVA) pathway in eukaryotes or the methylerythritol-phosphate (MEP) pathway in prokaryotes and plant plastids。However, through parts BBa_K5000001, we introduce a new pathway to produce IPP and DMAPP, most importantly it allows us decouple isoprenoid biosynthesis from central carbon metabolism, and has a very high flux.

This project is very similar with us. They aim to develop biobricked pathway expressing all necessary genes for rebaudioside A production in common baker's yeast Saccharomyces cerevisae. They overexpress certain key enzymes in order to increase the steviol biosynthesis. We express two new glycosyl transferase UGT91D2w and EUGT11M(F379A) to produce rebaudioside D and rebaudioside M which have a much better taste.