| Number | Name | Description |
|---|---|---|
| BBa_K4594011 | PKaiBC | Output promoter of the internal rhythm of Synechococcus elongatus PCC7942. |
| BBa_K4594010 | NSIII-2 | Neutral site 3(NSIII)is one of the insertion sites commonly used in the transformation of Synechococcus elongatus PCC7942. NSIII-2 acts as an upstream homology arm. |
| BBa_K4594009 | NSIII-1 | Neutral site 3(NSIII)is one of the insertion sites commonly used in the transformation of Synechococcus elongatus PCC7942. NSIII-1 acts as a downstream homology arm. |
| BBa_K4594015 | NSII | Neutral site 2(NSII) is another insertion site commonly used in transforming Synechococcus elongatus PCC7942. We plan to obtain this sequence from PCC7942 by PCR and use it for subsequent plasmid construction. |
| BBa_K4594016 | glgc sRNA | glgc sRNA binds to the mRNA of the D-glucose-6 phosphotransferase gene glgc and prevents its translation with the help of additional scaffolding hairpin structures and Hfq proteins. |
| BBa_K4594012 | PDHC | The coding sequence of pyruvate dehydrogenase PDHC in PCC7942. can increase its carbon flux and thus improve its ability to produce substances. |
| BBa_K4594018 | PpsbAI | PpsbAI is a constitutive strong promoter of PCC7942. |
| BBa_K4594017 | PnrsB-BCD | PnrsB has been identified as a promoter that responds most robustly to Ni(2+) ions. To optimize chances of efficient translation initiation, bicistronic design (BCD) was added to the promoter sequence to create PnrsB-BCD. |
| BBa_K3237026 | nucA | Nuclease, used for the killing switch. |
| BBa_K3237025 | nuiA | Nuclease inhibitor, used for the killing switch. |
| BBa_K4849000 | PrnpB | weak constitutive promoter |
| BBa_K4115001 | sfGFP with LVA | Superfolder GFP with an LVA degradation tag on its C terminal. |
| BBa_K4115012 | lac UV5 promoter | A variant of lac promoter, which does not depend on the activation of CRP. The lac operator is included in this part. |
| BBa_K4115006 | lacI | The lacI promoter, lacI CDS and a part of pET backbone containing the terminator of lacI are included in this part. |
| BBa_K196300 | Hfq | Hfq is an RNA-binding protein. It can bind to a range of RNA sequences but is often used in conjunction with an RNA hairpin formed by the E. coli micC sequence. A biobrick containing a template for making sRNA fusions to micC has also been designed BBa_K1963002. |
| BBa_K4594002 | luxF | luxF is a gene coding the protein LuxF, acting as a scavenger of myrFMN to activate luciferases. |
| BBa_K4594003 | luxG | luxG is a gene coding FMN reductase, providing substrate FMNH2 for luciferases. |
| BBa_K4594005 | cp157Venus | One circularly permuted Venus variant was improved by Takeharu Nagai, a Venus with a better BRET effect. |
| BBa_K4594006 | LuxB:cp157Venus | The fusion protein is formed by linking cp157Venus to the C-terminus of LuxB by Glu Leu linker. BRET can occur between LuxB and cp157Venus, changing the wavelength while producing brighter light. For cp157Venus please refer to BBa_K4594005, for LuxB please refer to BBa_K3769013. |
| BBa_K3769012 | LuxA | It can bind with luxB become luxAB.LuxAB is a luciferase which can utilize the aldehyde made by LuxCDE to produce blue-green light. Sequence and Features |
| BBa_K3769013 | LuxB | It can bind with luxA become luxAB.LuxAB is a luciferase which can utilize the aldehyde made by LuxCDE to produce blue-green light. Sequence and Features |
| BBa_K4594007 | A1 | One of the brightest fluorescent proteins in the FPbase database has an excitation wavelength of 490-520 nm and an emission wavelength of 525 nm. |
| BBa_K4594008 | A2 | One of the brightest fluorescent proteins in the FPbase database has an excitation wavelength of 490-520 nm and an emission wavelength of 512 nm. |
| BBa_K4594026 | B2 | A fluorescent protein with only amino acid sequence information in the FPbase database. The LSTM system inferred that the excitation wavelength of this fluorescent protein is between 490 and 520 nm, and its excitation wavelength is 506nm， which was trained by the ShanghaiTech iGEM team in 2023. |
| BBa_K4594028 | B2 | A fluorescent protein with only amino acid sequence information in the FPbase database. The LSTM system inferred that the excitation wavelength of this fluorescent protein is between 490 and 520 nm, and its excitation wavelength is 506nm， which was trained by the ShanghaiTech iGEM team in 2023. |
| BBa_K4594028 | C1 | Artificially generated fluorescent protein. The fluorescent protein was generated by the Generative Adversarial Network and the excitation wavelength of this fluorescent protein was inferred to be between 490 and 520 nm by the LSTM system. The protein was generated by the ShanghaiTech iGEM team in 2023 and its luminescence is currently unproven. |
| BBa_K4594029 | C2 | Artificially generated fluorescent protein. The fluorescent protein was generated by the Generative Adversarial Network and the excitation wavelength of this fluorescent protein was inferred to be between 490 and 520 nm by the LSTM system. The protein was generated by the ShanghaiTech iGEM team in 2023 and its luminescence is currently unproven. |
| BBa_K4594030 | LuxB:A1 | A fusion protein formed by linking the A1 fluorescent protein to the back of the LuxB fluorescent protein with a Glu-Leu linker. For A1 please refer to BBa_K4594007. For LuxB, please refer to BBa_K3769013. |
| BBa_K4594031 | LuxB:A2 | A fusion protein formed by linking the A2 fluorescent protein to the back of the LuxB fluorescent protein with a Glu-Leu linker. For A2 please refer to BBa_K4594008. For LuxB, please refer to BBa_K3769013. |
| BBa_K4594032 | LuxB:B1 | A fusion protein formed by linking the B1 fluorescent protein to the back of the LuxB fluorescent protein with a Glu-Leu linker. For B1 please refer to BBa_K4594026. For LuxB, please refer to BBa_K3769013. |
| BBa_K4594033 | LuxB:B2 | A fusion protein formed by linking the B2 fluorescent protein to the back of the LuxB fluorescent protein with a Glu-Leu linker. For B2 please refer to BBa_K4594027. For LuxB, please refer to BBa_K3769013. |
| Number | Name | Description | Basic Parts |
|---|---|---|---|
| BBa_K4115045 | NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1 | This part is used to insert a cscB expression box into the genome of S.elongatus. | |
| BBa_K4594004 | LuxC-D-A-B-E-G-F | The composite part combines J70008, BBa_K4594003, BBa_K4594002 | [BBa_J70008]-[BBa_K4594003]-[BBa_K4594002] |
| BBa_K4594019 | NSIII-2-PkaiBC-sfGFP-lacI-KanR-NSIII-1 | The sfGFP in this part has a degradation label and is expressed under the control of PkaiBC. At the same time, it can be inserted into the genome of Synechococcus elongatus PCC7942 through NSIII. Therefore, this part can be used to verify the feasibility of the rhythmic expression of foreign genes in PCC7942. | [BBa_K4594010]-[BBa_K4594011]-[BBa_K4115001]-[BBa_K4115006]-[BBa_P1003]-[BBa_K4594009] |
| BBa_K4594020 | NSIII-2-PkaiBC-LuxC-D-A-B-E-G-F-KanR-NSIII-1 | The Lux gene cluster in this component has already been proven to emit stronger fluorescence in Escherichia coli and is expressed under the control of PkaiBC. Additionally, it can be inserted into the genome of Synechococcus elongatus PCC7942 via NSIII. Therefore, this component can be used to enable PCC7942 to rhythmically emit strong fluorescence. | [BBa_K4594010]-[BBa_K4594011]-[BBa_K4594004]-[BBa_K4115006]-[BBa_P1003]-[BBa_K4594009] |
| BBa_K4594021 | NSIII-2-PkaiBC-LuxA-LuxB-PpsbAI-LuxC-LuxD-LuxE-LuxG-LuxF-KanR-NSIII-1 | This component is based on BBaBBa_K4594020 and splits the LuxCDABEGF gene cluster. The LuxCDEGF genes responsible for synthesizing the fluorescence substrate are under the control of the strong promoter PpsbAI, leading to increased substrate synthesis during the day, further enhancing its luminescence intensity at night. | |
| BBa_K4594022 | NSII-1-PnrsB-BCD-nuiA-PrnpB-nucA-KanR-NSII-2 | This component is a Ni ion-dependent suicide switch. The nuclease (nucA) is continuously expressed under the control of a weak constitutive promoter. In a closed culture environment with Ni ions present, the PnrsB-BCD promoter is induced, leading to the expression of the nuclease inhibitor (nuiA), counteracting the self-toxicity of the nuclease. However, when engineered algae are released, the Ni ion concentration decreases, nuiA is not expressed, and the nuclease exerts its self-toxicity, killing the engineered algae. | [BBa_K4594015]-[BBa_K4594017]-[BBa_K3237025]-[BBa_K4849000]-[BBa_K3237026]-[BBa_P1003] |
| BBa_K4594013 | LuxA-LuxB | LuxA and LuxB proteins form a dimer that catalyzes the oxidation of fatty aldehydes to fatty acids, while reducing FMNH2, producing light with a wavelength of 490nm. | [BBa_K3769012]-[BBa_K3769013] |
| BBa_K4594014 | LuxA-LuxB:cp157Venus | cp157Venus can generate BRET effect with the dimers of LuxA and LuxB, and emit light with a wavelength of 528nm. | [BBa_K3769012]-[BBa_K4594006] |