March

3/4

  • Topic decision 
  • Information collection

3/11

  • 7 chemical decided: Astaxanthin, NMN, PQQ, Vit B6, Butyrate, HMB(β-Hydroxy β-methylbutyric acid), KIC(α-Ketoisocaproic acid) 
  • The seven chemicals’ effects on human 
  • mTOR signal transduction pathway research

3/18

  • Market analyzing of the Sarcopenia 
  •  Data collection on Sarcopenia 
  • Research on supportive treatment of Sarcopenia

3/25

  • Research on the cause of the Sarcopenia
  • Research on the mechanism of Butyrate and HMB to boost muscle production
  • Deeper research in mTOR pathway

April

4/1

  • The connection of mTOR and Butyrate and HMB
  • Research the mechanism of boosting muscle of other compounds:Astaxanthin, NMN, PQQ, Vit B6,and KIC
  • Creating 31 pages report for our research

4/8

  • The connection of mTOR and Butyrate and HMB
  • Research the mechanism of boosting muscle of other compounds:Astaxanthin, NMN, PQQ, Vit B6,and KIC
  • Creating a 31 page report for our research on 7 chemical compounds

4/15

  • Decide the organism of producing these compounds
  • Recruit new members
  • Creating introductory presentation on PPT
  • Done with our drawing of chemical compounds

4/22

  • Distribute the works of producing chemical compounds to three probiotics 
    1.Clostridium Butyricum: Butyrate, Vitamin B6 
    2.Bacillus subtilis: PQQ, HMB, KIC 
    3.Kluyveromyces marxianus: NMN, Astaxanthin
  • Looking for metabolic pathways for producing these compounds on KEGG or other resource
  • Done the First draft on metabolic pathway producing NMN

4/29

  • Decide the knockouts of the genes
  • Starting to search for possible kill switch

May

5/13

  • Decide to concentrate on NMN,HMB,and KIC production
  • Done the second draft of NMN producing pathway

5/20

  • Done the final draft of NMN producing pathway
  • Designed our own pathway for NMN production

5/27

  • Interview with doctors
  • Kill switch design by using operon system
  • Learned on biobrick build up

June

6/3

  • Research on the conformation of the enzyme NAMPT(an enzyme essential for NMN)
  • BLAST and search for the highest efficiency of NAMPT to produce most NMN
  • Finalize the pathway to produce HMB and KIC

6/10

  • Keep researching on NAMPT
  • Researching for the Intestine environment and the location of our probiotics will grow
  • Researching for dosage of our chemical compounds

6/17

  • Finding out sequence for what we want to insert and what we want to knock out in our metabolic pathway
  • Design the second draft of our kill switch for Bacillus Subtilis

6/24

  • Finding out key active site of the NAMPT
  • Design the third draft of our kill switch for Bacillus Subtilis
  • Research for hydrogen sulfide sensitive repressor
  • Finding Toxin and Antitoxin of Bacillus Subtilis

July

7/1

  • Draw out the kill switch for Bacillus Subtilis
  • Drawing the first draft of kill switch of Clostridium and kluyveromyces

7/8

  • Choosing the expression vector of Bacillus subtilis
  • Preparing for competent cells
  • Draw out the gene map

7/15

  • Learning to use snapgene to draw map
  • Design the map of kluyveromyces marxianus

7/22

  • Send out the order of fragments
  • Sequence optimization
  • Optimize the design

7/29

  • Finalize the genes and order out
  • Complete the transformation of TDH3,ADH1 promoter and GFP gene from the distribution kit
  • Done the colon PCR

August

8/5

  • Designing experiments

8/12

  • Start the experiments of NadA,NadB,and NadE fragments
  • Ligation and transformation for the plasmid
  • Gel extraction
  • Check the length of pKlac2 plasmid

8/19

  • Colony PCR checking
  • Failed the ligation

8/26

  • Started Astaxanthin experiment
  • Planning to redo the NMN experiment

September

9/2

  • Wiki build up- HP and wet lab
  • Final presentation making

9/9

  • Wiki build up- Projet,wet lab, and Human practice
  • Enter register
  • Started making promotion videos

9/16

  • Wiki build up- Projet,wet lab, and Human practice
  • Checking for safety forms
  • Finalize promotion videos

9/23

  • Wiki build up- Projet,wet lab, and Human practice
  • Finalizing safety forms
  • Finalize promotion videos