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Medal

Medal

小桌宠


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    In 2023, the OUC-China iGEM team utilized a newly discovered fungus, M. aphidis XM01 , from the mangrove forests in Hainan, China, to regulate its metabolic pathways. They engineered this organism to produce medium-chain fatty acids and expressed the corresponding enzymes to synthesize medium-chain α-olefins and 10-hydroxydecanoic acid. These developments hold significant industrial value.

    Upon recognizing the challenge of removing benzo[a]pyrene commonly found in kitchen waste oil, the team designed a light-controlled circuit for its degradation. Finally, they implemented a NeuAc riboswitch as a safety measure to prevent gene leakage, enhancing the project's security.The project was divided into three main parts: Medium Chain Fatty Acid Derivatives Synthesis System: This part focused on regulating the metabolic pathways to produce medium-chain fatty acids and related derivatives. Benzopyrene Degradation System: Here, a system was designed to degrade benzo[a]pyrene, a challenging compound often found in kitchen waste oil. Killing System: This component was responsible for implementing the NeuAc riboswitch to prevent gene leakage and enhance project safety.



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    1.Deliverable

    We successfully submitted the wiki, project promotion video, presentation video,Judging form and completed the judging Sessions.

    2.Attributions

    We used standardized forms to describe the attributions of team members and others to our projects.More information about Attribution can be found on the Attribution page of our Wiki.

    Link for Attributions

    3.Description

    We describe why we chose this project, explain the significance of using our knowledge of synthetic biology to solve this problem, and outline our goals and ways to achieve them.More information about Project description can be found on the description page of Wiki.

    Link for Description

    4.Contribution

    We made a useful contribution to the future iGEM team by recording video of some key experimental operations on the b site, designing new parts, and designing new hardware related to fermentation, printing it using 3D printing technology, and documenting the production process.More information about contribution can be found on the Contribution page of Wiki.

    Link for Contribution



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    1.Engineer success

    We carried out the iteration of the engineering design cycle.We carried out the steps of design → build → test → learn, designed the new part, measured the performance of the new part, and gave the idea of the subsequent improvement scheme.More information about Engineer success can be found on the Engineer success page of our Wiki.

    Link for Engineering

    2.Human practice

    We design our projects with environmental concerns in mind, using kitchen waste oil to produce our target products. We have designed a questionnaire on kitchen waste oil, and actively interviewed government personnel and relevant professionals to understand the current situation of recycling and utilization of kitchen waste oil, and obtain the public's views and expectations on our use of kitchen waste oil to produce medium-chain fatty acids and other products, so as to further improve our project. We actively explore the boundaries of the project, constantly communicate with the audience of the project, modify the project according to their feedback, and explore the conditions for the product to be truly applied.More information about Human practice can be found on the Human practice page of our Wiki.

    Link for Human-practice



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