Experiments

Overlap PCR

DNA Cloning

Golden Gate Assembly

PCR Clean-Up

Preparation of Agarose Gel

Preparation of Component Cells (10Beta E.Coli Strain)

Plasmid Extraction (Plasmid Isolation)

DNA Isolation (Gel Clean-up)

Polymerase Chain Reaction (PCR)

Gel Electrophoresis

Colony PCR

Error-prone PCR (Random Mutagenesis)

Oligo Pool Resuspension and PCR

GFP-sacB Characterization