Our parts prove to be comparable to the PE2/PE3 system, providing future iGEM teams an alternative option for prime editing. The underlying potential using prototype foamy virus reverse transcriptase (PFV RT) as a highly compatible RT to prime editing also remains undiscovered. Successful prime editing using the PFV RT constructs in stem cells also open new possibilities to therapeutic targets and interventions. These parts can be used by future teams for application in prime editing from generating specific gene knockouts to possible therapeutic interventions.

Two stem cell lines (H9, ACS1030) were tested in the project and optimisation of transfection using Lipofectamine 3000 was carried out. These results can be used in future team with ease without re-optimisation for the cell lines.