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1.Our team has also added new documentation to an existing Part on that Part's Registry page (BBa_K3384313 and BBa_K3384314). We add not only new data collected from laboratory experiments, but also the new information learned from literatures.



We collaborated with Team NJtech_China 2019 and obtained strains BY4741 pRS415-prm1 Pro-GFP-CYC1(BBa_K3384313)and BY4741 pRS415-prm1 Ultra-GFP-CYC1 (BBa_K3384314). We used a flow cytometer to characterize the fluorescence expression intensity of BBa_K3384313 treated with 0μM-1.5μM α-pheromone and BBa_K3384314 treated with 0μM-5μM α-pheromone. Since the flow cytometer is expensive, we cooperated with team members of NJtech_China 2019, who performed flow up-sampling and sent the obtained data to us, and we performed flow data processing (using flowjo, Graphpad). The results were as follows:

(1)The fluorescence expression intensity of BY4741 pRS415-prm1 Pro-GFP-CYC1 (BBa_K3384313) under 0μM-1.5μM α-pheromone treatment was characterized using flow cytometry. The data reveals that with α-pheromone treatment ranging from 0μM to 1.5μM, the fluorescence intensity rises proportionally alongside the concentration of α-pheromone. This relationship follows a linear pattern, suggesting the possibility of designing it as a rheostat.

Fig.1 Characterization of the fluorescence intensity of BY4741 pRS415-prm1 Pro-GFP-CYC1 (BBa_K3384313) by using flowjo software.

Fig.2 Characterization of the fluorescence intensity of BY4741 pRS415-prm1 Pro-GFP-CYC1 (BBa_K3384313) under 0μM-1.5μM α-pheromone treatment by using prism software.


(2).The fluorescence expression intensity of BY4741 pRS415-prm1 Ultra-GFP-CYC1 (BBa_K3384314) was characterized using flow cytometry under of 0μM-5μM α-pheromone treatment. The data shows that with α-pheromone treatment ranging from 0μM to 5μM, the fluorescence intensity remains relatively constant despite the increase in α-pheromone concentration. This characteristic could be harnessed for a switch design.

Fig.3 Characterization of the fluorescence intensity of BY4741 pRS415-prm1 Ultra-GFP-CYC1 (BBa_K3384314) by using flowjo software.

Fig.4 Characterization of the fluorescence intensity of BY4741 pRS415-prm1 Ultra-GFP-CYC1 (BBa_K3384314) under 0μM-1.5μM α-pheromone treatment by using prism software.




2.After four cycles of DBTL, our team has contributed to iGEM Registry by adding new composite parts (BBa_K4779000, BBa_K4779001, BBa_K4779002, BBa_K4779003) and three new basic parts (BBa_K4779004, BBa_K4779005, BBa_K4779006).



As is shown in the following table.