Experimental results
Construction of NOG pathway strain in Clostridium tyrobutyricum
Figure 5 Nucleic acid gel verification of engineering Clostridium Ct ( FXpk ) recombinant plasmids (a. FXpk fragment
derived from Bifidobacterium adolescent b. FXpk fragment derived from Clostridium acetobutylicum c. Linear vector X- pthl )
Figure 6 Comparison of Growth Performance of Engineering Strains Ct( FXpk -QS ) and Ct( FXpk -BD )
|
strain
|
Glucose content (g/L)
|
Acetic acid (g/L)
|
Butyric acid (g/L)
|
|
control
|
0.82±0.82
|
1.43±0.21
|
3.06±0.24
|
|
Acetin source engineering bacteria
|
0
|
1.36±0.26
|
3.3 5 ±0.22
|
|
blue double source engineering bacteria
|
0.09±0.09
|
1.28±0.24
|
3.3 1 ±0.24
|
Table 3 Product comparison of engineering strains Ct ( FXpk -QS ) and Ct ( FXpk -BD )
According to the growth curve in Figure 6, it can be seen that the growth of the engineering bacteria derived from Clostridium
acetobutylicum is better than that of the engineered bacteria derived from Bifidobacterium adolescentis. From the product
analysis in Figure 7, it can be found that the butyric acid production of the engineered bacteria derived from acetone butanol
has increased by 9.5% compared with the control. In addition, the production of by-product acetic acid decreased from 1.43 g /L
to 1.36 g/L, it can be concluded that the phosphoketolase derived from Clostridium acetobutylicum is a better strain.