Global warming, rising sea levels, and continued environmental degradation are all linked to carbon emissions. In China, microbial carbon
emissions account for 7% of the total, and most of the carbon emissions generated by microorganisms come from sugar degradation. Carbon
sequestration is conducive to reducing carbon emissions. This project attempts to introduce a highly active phosphoketolase (F/Xpk) gene
into the heterotrophic C. tyrobutyricum, to construct an artificial NOG pathway with high metabolic flux. Compared with the native EMP
pathway, this alternative pathway metabolizes glucose into acetyl CoA without carbon loss. We further tried to improve the F/Xpk expression
by promoter engineering and ASR. Besides, we found that this engineered strain has a higher ability to utilize cheaper carbon sources. The
approach used in this project can be further adapted to a variety of strains with similar metabolic carbon emission issues, thereby
contributing to the reduction of global carbon emissions.