Human Practices

"Human Practices is the study of how your work affects the world, and how the world affects your work."

— Peter Carr

🙉 Scroll to See Stage at a Glance

📘 Overview

    This year, we will create a Living Building Material (LBM), a sand-hydrogel scaffold with Cyanobacteria.  Our ultimate aim is to generate an effective Bioconcrete-generation mechanism and product that successfully substitutes traditional concrete in consolidating building structure. Upon this basis, we could implement entrepreneurship and community-oriented investigation for our project, which includes a collection of different social interactions running through all 5 stages, maintaining robust and mutual connections with the world in all time. We engaged communities involved in the problem in different phases, such as producers, manufacturers, researchers, non-profit, disposal-related people, user, business development experts, and the general public. When we were designing the project, multiple values were practiced, including environmental sustainability, social engagement, scientific inquiry, and moral responsibility.
    We initially saw many concerns expressed by the public regarding the feasibility, we thereby saw the need to conduct large-scale public education and communication as our project continues to mature. This value of listening emphasizes the importance of actively listening to the community's feedback and engaging in open dialogue to ensure the project's transparency and responsiveness to real-world challenges.
    Below is our Human Practice journey that constructed a conversation with the world.

👀 1. Find out a problem

Students’ Brainstorm

    Our approach to human practice is guided by our goal of addressing real-world challenges. We aim to begin by addressing local issues to make our approach more relatable to our audience. In the first phase, our team was able to brainstorm different ideas through not only comprehensive research but also by tapping into personal experiences. Our teammembers were able to use personal experiences, such as cat urine in local communities proposed by Dorothy, as she is bothered by this problem in her home compound, to demonstrate our approaches toward local issues. Another example of the usage of local issues is an idean on bio concrete. Here, we hoped to solve building cracks during earthquakes as well as the damage of traditional concrete, initially inspired by our students’ notice towards the outrageous proportion of CO2 concrete production releases in her local area (responsible for 8% in all global CO2 emissions by category, and especially the huge role China plays in producing it). Additionally, recent events like the Turkey-Syria earthquakes in 2023 further attracted our attention, seeing the need for earthquake-resistant construction materials with lower environmental impact.
Ideas in the Brainstorm Pot
Grouped into Three Categories
    One by one, our team generated a variety of ideas, including skin whitening products, bio-concrete, DNA data storage, diabetes solutions, cat urine disposal, gut microbiota, biodegradable dyes, and the use of plant volatile organic compounds in food preservation, among others. In addition to individual brainstorming, we established three distinct teams to further categorize and expand upon these initial brainstormed ideas: the Environmental Protection Group, the Daily Needs Group, and the Food Nutrition Group.
Exemplar case studies presentations on these topics

Three Groups
    Moving on to the second stage, we engaged in numerous discussions, and gradually narrowed down our ideas to two main topics: spermidine and bio-concrete. This progression laid the foundation for our second and third stage, during which we focused our research specifically on bio-concrete.

💭 2. Weighing the Problem: Human Impact

Public Survey

Context:
    After finding out the problems of current building material and spermidine production, we decided to delve deeper into them and see if they truly possess valid social and technological feasibility and a potential to reshape the shared future of the world we live in. The best way to do this is by asking the ultimate audience and possible instructors of our work––the public and experts. In the second stage of our Human Practices, we seek for robust justifications for our new-born project ideas through investigation based upon surveys and interviews that reflect the society’s attitude toward our project ideas. The feedback we collected helped us to make our final decision on which project to build upon in the rest of the year.
    The first step we made in this stage is conducting a public survey on the two potential project ideas we came up with, which would let us see how the general public think of the two ideas and what questions they might raise. This becomes crucial in our decision of project idea elimination.
Rationale:
    When it comes to social investigation, survey is an effective tool that collects responses. In the feedback of the survey, we can clearly compare and contrast the two project ideas in terms of their social and technological feasibility and the concerns they raise. The more need the respondents show toward a project, the more likely we are going to adopt it at the end.
What we learned:
    Through our survey, we gauged public opinion on our idea and the two problems at hand. We also explored the need for potential solutions.
    Our findings revealed that many individuals possess creative and reliable solutions for spermidine-related challenges. However, they generally seem content with the status quo, perceiving the adoption of bio-engineered spermidine as costlier due to factors such as risk, human resources, and time. Moreover, they do not view resolving spermidine issues as having a significant impact on people's lives since it is not considered a necessity for most individuals. Many respondents also indicated that they had never encountered or used spermidine before. These insights prompted us to reevaluate the significance of the problem we were addressing. We realized that focusing on concrete, a material encountered by nearly everyone daily, could have a more substantial impact.
    Despite the familiarity of respondents with concrete, they were unaware of bioconcrete solutions. This observation aligns with our understanding that many Chinese citizens have limited exposure to sustainable building materials in their homes. Conversely, respondents from other countries mentioned that many households there opt for sustainable sources and seek information from websites like "Conserve Energy Future." These responses also demonstrated in-depth knowledge of analyzing a building's carbon footprint and implementing measures to reduce it.
Public Survey Results
      In summary, our survey confirmed the need for a solution, leading us to establish two primary goals for our project:
  • Mitigating global warming through sustainable technology integration
  • Increasing local awareness of this problem by educating the public about biotechnology and our project's significance. We intend to achieve the latter through comprehensive efforts in public education.


  • Interview with Dr. Chen

    Context:
      We wish to roughly confirm the bioengineering feasibility first. After conducting a public survey, our team spent a month in deep research and discussions. We've outlined a potential engineering design for producing bio-concrete using Cyanobacteria Synechocystis pcc6803, including biological chassis, enzymes, and chemical mechanisms. While we've clarified the scientific principles to form a bioconcrete with the help of cyanobacteria, and created a preliminary plan for plasmid designs, many crucial implementation details, like the co-culture system's feasibility, Synechocystis sp. survival conditions, and the surface display mechanism, remain uncertain and can't be determined from literature alone. Therefore, professional guidance is essential to partially evaluate and finalize our experimental designs and methodologies at this stage.
    Interview to Doctor Chen
      We wish to receive professional advice from a researcher working with the model organism of cyanobacteria to evaluate the feasibility of our preliminary experimental designs and to suggest improvements that can be made to our experimental design. This will help our team to achieve the production of bio-concrete with the optimal method.
      With this purpose in mind, we interviewed Dr. Chen, a biology researcher specializing in work with cyanobacteria. Dr. Chen received his bachelor’s degree at Tsinghua University, received his Doctorate degree from Tianjin University, and is currently a Post-Doctorate in Shenzhen University.
    What we learned:
      Since we want our product to be drought-tolerant, there are a few aspects that Dr. Chen suggested we do. We may find drought-tolerant cyanobacteria at the start, such as transferring the gene of desert cyanobacteria or overexpressing the anti-osmotic substance. There are also algae that may lose water and live again with water that we may use.
      Dr. Chen also gives some advice on the system we are using. First, the algal-bacterial symbiotic system will have problems with the two bacteria's survival and growth. We may need to do more research and rethink the system since a single-cell system may also work for our project. we are using cyanobacteria for the process; Dr. Chen points out that it will take up a long experimental process. Glucose may be used to speed up, but mold will also grow very fast and cover up our bacteria.

    🛫 3. Studying the Technicals of Concrete


    1️⃣ Life Cycle 1: Producer (Extraction, Production)

    China West Construction Group Co., LTD

    Context:
      As reading different relevant literature went on, we at the time were able to understand concrete roughly, and had an idea of using cyanobacteria. In last stage, we have roughly first confirmed that our potential bioengineering of cyanobacteria is doable with the help of a cyanobacteria-specialist. In this stage, we wish to further understand concrete life cycle and its technical mechanism to better see how this bioengineered thing can kick in to help and pertain to its current usage and help adapt and transition production methods. This largely relies on consulting some large construction and real estate corporations whose services are closely related to the use of building material. Our interview with China West Construction Group is an example of this type of practice.
      Our goal is to explore the major players in the concrete supply chain and interview each one to create a comprehensive narrative of the entire supply chain.
    Reaching Out:
      China West Construction Group is a pioneering service provider of building materials in the construction industry in China. We learned about China West Construction Group in news and contacted them via e-mail.
      Click here to learn more about China West Construction Group.
      We tailored a list of interview questions that is specific to the key points we hope to learn about concrete before we planned interview sessions with construction companies. These questions guided us to have in-depth conversations with the leaders in the construction industry, and prompted us to rethink about our project’s engineering. If we hope to substitute traditional concrete with novel bio-based building material, we have to be 100% sure about current traditional concrete’s cost, functions and attributes, and interview with construction company who produces traditional concrete establishes a credible source of information.
    Interview with West Construction Group
      We wish to receive professional advice from a researcher working with the model organism of cyanobacteria to evaluate the feasibility of our preliminary experimental designs and to suggest improvements that can be made to our experimental design. This will help our team to achieve the production of bio-concrete with the optimal method.
    What We Have Learned
      From the meeting, we learned about the current requirements in the concrete industry for concrete in general. We learned about what we needed to do to take our bioconcrete up to that level. In China, there is a series of requirements that are named 864 requirements. These include standards on how to create the concrete, what amount of aggregates to use, what amount of sand and rocks to use, and how to mix it all together etc.. These also include standards on compressive strength etc. After the interview, we were able to look for this so we could better design our testing system when working in the wet lab.
      Other than this, we also learned about the need for our solution. We learned that though some companies were worried about the strength problems of bioconcrete. Companies also tried their best to decrease the amount of CO2 emission from traditional concrete to fit the country’s guidelines. However, sometimes, CO2 would leak from their factories. They have also tried to optimize their ratios so there is less cement in the concrete. However, the most they can do is decrease the use of cement from 200 kg to 180 kg per one block of concrete. This still isn’t the most optimal solution. They can’t continue to optimize this since cement is the most important material that acts like a strong glue.
      Also, though there isn’t a great need for a biobrick, there is a great need for biosealent in these companies. Concrete can crack easily if it isn’t set properly because of errosion or temperature changes. Once it cracks, it’s really hard to repair.
      Finally, his attitude towards our project was that most green solutions are still very young and they often have problems on safety and effectiveness or cost. They do use them at times, however, they normally use them with traditional concrete together and not to replace them. This is because they are worried about the aforementioned problems. Hence, if there is indeed a green solution that doesn’t have these problems, they would be happy to use it.


    2️⃣ Life Cycle 2: User (Business Construction)

    Zhenye Co., LTD

    Context:
      Our interview with company Zhenye is another example of this consulting some large construction and real estate corporations whose services are closely related to the use of building material in its supply chain.
      This time, we spoke with Zhenye Company, a real estate company that purchases and constructs buildings using concrete from other companies, acting as a customer. Zhenye is a well-known and established company in China, founded in 1989, and became an IPO company in 1992.
      During the planning stage of our project, we need to conduct extensive research on the market for our bio-concrete. We interviewed the CEO of the company, Mr. Cai Zhen, who is also the founder.
    Speaking to the CEO of the real estate company
    Reaching out:
      Firstly, we want to understand the factors that influence their choice of concrete. This will help us establish success criteria for our own product, as our aim is to bring our product to the market. Secondly, we want to learn how consumers use concrete in practical applications, which will assist us in designing our concrete to better meet their needs. Lastly, we want to understand the real estate company's attitude towards using "living concrete" in their buildings, which will provide us with information for future public education activities.
    What we learned:
    • Mr. Cai provided us with detailed data on the proportion of concrete materials in a building, emphasizing the importance of concrete in modern construction. He stated that "concrete, as the most popular building material, is durable, stable, and cost-effective. It is an irreplaceable material in modern construction." This information solidified the concept that concrete is the most popular building material in the market today. It confirmed that our project has a relatively wide market in the field of construction.
    • Additionally, Mr. Cai mentioned that quality is always his company's top consideration when choosing concrete, and sustainability is another important criterion. He highlighted that the sustainability department of his company strictly controls waste management. This information reminded us of the need to ensure the quality of our bio-concrete and inspired us to design different performance tests for our product. It also reinforced our belief that sustainability plays a crucial role in modern construction.
    • Furthermore, when we introduced our bio-concrete to Mr. Cai, he expressed willingness to use our product if it passes performance tests and demonstrates clear advantages over traditional concrete. He suggested that bio-concrete could reduce waste in the production process and increase the durability of the concrete. This information motivated us to focus on sustainability as our competitive advantage and strive to surpass traditional concrete.
    • Advisor Emma Participating in the Interview


      3️⃣ Life Cycle 3: Disposal Related People (Repair & Recycle)

      Beijing Zhongye Baocheng Building Restoration Technology Co., Ltd.

      Context:
        The previous two companies helped us a lot in understanding the technicalities of concrete production, giving us a full picture of its production methods and environmental impacts brought. We then wish to engage in activities that would reinforce our understanding of the disposal, reparation of concrete, since we understood that concrete could deteriorate depending on its different production methods, implying the fact that frequent renewal, reparation, recycling are actually involved. This process of having short-lived concrete replaced adds on our concern that the process of instability and more environmental impacts. We then did research online, hoping to reach to a concrete-reparing company. Luckily, we were able to contact a typical building restoration company in China through their website’s phone numbers.

      The Restoration Company's Service & Products

        "Beijing Zhongye Baocheng Building Restoration Technology Co., Ltd. is a professional organization specializing in concrete crack treatment, concrete defect repair, concrete durability restoration and protection, crack investigation, and diagnostics, with its headquarters located in Beijing."
        Through the phone call, we were introduced to their major job descriptions and a few examples of concrete investigation and crack treatment they did before, and how we can hope to reach out to them if we have any specific crack in concrete that needs their service.


      4️⃣ Life Cycle 4: Non-Profit Oriented Support

      Context:

      We also recognize the presence of numerous nonprofit organizations with a sustainability focus that actively embrace state-of-the-art solutions, primarily through technological advancements and creative problem-solving approaches. These organizations often harness various technological methods, with a particular emphasis on those rooted in biological and environmental sciences. Consequently, we have identified a strong alignment with these organizations and believe they would be receptive to our ideas. We are keen to learn from them regarding concrete pollution and their insights into potential new solutions.

    We reached out to a mature nonprofit that focuses on ecology and environment, called "Operation Earth Environmental Expedition". It is basically a nonprofit platform provided to youths to experience field trips in different ecological environment and learn experentially, and also host panel discussions and other educational contents. We reached out to its Founder, Ms. Ma, for their perspective on the issue and their attitude towards our project. After scheduling the first meeting, Ms. Ma said she would love to connect us with an expert in their board for more information, and suggested us to do a proposal, or a question list to send to the expert. We then did so, and it was a very valuable experience as they shared about information on the projects they did with bio-based materials, and how governmental incentive had an impact on business decisons.

    We sent our proposal to Mr. Wang Zhidong, an expert in Photovoltaic Architecture at China Construction Technology Group Co., Ltd. In 1994, he transferred to China Architecture Science Research Institute, where he engaged in work related to air conditioning, HVAC, purification, energy-saving technologies, and engineering. Since 2013, his focus has shifted towards the application of photovoltaics in buildings, green architecture, and building energy efficiency. Mr. Wang also served as the Executive Director of the Fire Protection and Comprehensive Technology Branch of the Architectural Society of China.


    A List of Question/Proposal to Inquire Nonprofit Support

    Articles they wrote on their Wechat Account. Please note that this is auto-translated. The coherency and formatting may be incoherent sometimes.



    🧑🏻‍🍳 4.Implementing a Solution: A Detailed plan


    👷🏻 Political constrains

    Reading and Summarizing National Regulations on Green Building Materials

    To understand the national policies and regulations on this matter, we think studying deeply and researching deeply is a better way to comprehend the problem rather than doing an interview. Hence, we have a group of students together reading and summarizing the national regulation of green building material.

    National Regulations on Green Building Materials (https://wwwgov.cn/zhengcezhengceku/2022-10/25/5721569/files5db7116352bb4b298f89df3ffe673cd0.pdf)

    Below is the presentation the group of students did and presented to the team to help us better understand.

    Guidelines Summary


    💸 Market Potential Constraints

    First Interview with Link Spider Co., Ltd

    CEO(left) and CTO(right) of Link Spider
    Context:
      Of the many Human Practices activities we conducted in the fourth stage that aimed to resolve the technological, economic, political and hardware constraints of our project, our interview with the CTO and CEO at Link Spider is perhaps the most meaningful one as it allows us to consult a real biotech company for knowledge relating to economic constraints. The interview provided us great insights into the entrepreneurship and commercialization of novel materials made by synthetic biology methods and guided us to think about the future development of our own product––is there a possibility for our bioconcrete to also enter the market?
    Rationale:
      There are a variety of factors that might limit a novel product’s successful commercialization, and the economic or financial constraint is a great one––how does the inventor of the product gather funds and support to initiate a successful entrepreneurship? Is there any possibility of requesting support from the government to help with the commercialization, given that the product is in the innovation and sustainability sector? With curiosity and concerns with these problems, we seek for an opportunity to consult a biotech company for experiences on entrepreneurship and finances. The feedback we receive can be substantial to the commercialization of our bioconcrete material, helping to send the product into the market and transform the building material industry.
    Reaching Out:
      Link Spider is a young biotech company that produces a range of bio-based materials made with synthetic biology, exemplified by the bio-fabricated leather made from spider silk protein. We contacted the founders of Link Spider to present our project idea and luckily arranged a virtual online interview session with them, centering on the commercialization and potential market of synthetic biology products in the current world.
      Click here to learn more about Link Spider.
      The CEO and CTO delved deep into the macro status quo of biotech industry in China, and portrayed Link Spider’s position in the market, and discussed about how they started their company together, their motivations, the lessons they learned, and the strategic shifts they took.
      However, what was most grateful was the real, practical, profound insights they shared. They provided us with concrete, detailed and practical examples from real-life experiences, including how how their company implemented corporate philosophy to its employees (they showed us their employee handbook), short and long term plans(including how frequent they re-fiance, how they identified their initial company narrative framework through the SDGs) , strategies (such as merging with traditional factories to mass produce the bioleather to lower production cost, thus getting rid of the costs that come along with building a niched factory catered for their bioleather production), to find a niche in the market.
      We raised some of the core concerns that troubles almost every bio-tech company, including questions like "what is your comparative advantage? How do you address high costs, given that these are ones of the most imperative challenges Biostone would encounter if we wish to commercialize the same way they do. They talked about how we should enhance the scalability of our product. They provided us with three steps:
    1. form collaboration with existing players in the traditional sector, opening for global resources and connections;
    2. build your own brand, forming collab with bigger brands, and display your technology on their products;
    3. gradually attract attention to your own brand. Moreover, they shared their journey with iGEM to illustrate us how iGEM inspired their company philosophy. The CTO at Link Spider was a member, an advisor, a coach for many years, and he told us that they got inspired by the “bottom-up approach” of iGEM, meaning looking from parts to system, and from the most basic responses from the public to developing a solution.
      We then consider ourselves lucky to have the opportunity to consult with the actual founders of a recently established biotech, as they are now experiencing the initial startup stage, meaning they are getting hands-on with both minor and imperative operations and outreach plans.

    Interview with Cargill

    Business Development Director Cargill Bioindustrial


    Context:
      Having successfully produced bio-concrete bricks and sealants in the lab, we're now looking to transition to real-world applications. Our recent discussion with LingZhu Technology provided valuable insights into the biomaterials market, but we require more specific information regarding biomaterials in the construction sector, especially in China's well-established concrete market.
      In pursuit of this knowledge, we recently interviewed the Head of Business Development in the bioindustrial sector at Cargill. Cargill, with over 150 years of history, primarily operates in agriculture and nutrition. Their bioindustrial sector offers a range of bio-based materials, including structural adhesives, alternative fuels, and polymer additives.
    Key Takeaways:
      The first is performance, the key: Cargill emphasized the paramount importance of performance for bio-based products. Given that bio-based products often have higher production costs, it's crucial that they outperform traditional alternatives. In response to this insight, we've conducted a cost analysis for our bio-brick, compared it to common brick types on the market, and considered ways to enhance our product's performance.
      The second is gaining Exposure. Cargill's leader offered valuable suggestions for gaining exposure for a bio-based product. This includes having a well-defined business strategy, seeking opportunities for exhibitions and product introductions, and conducting interviews with industry professionals. We've already started implementing these recommendations and have proposed activities at Keystone Academy to increase public awareness of our product.
      The third is market Resilience: Cargill's leader also highlighted a significant challenge for bio-based products: their ability to withstand market fluctuations and economic downturns. This raised the question of whether bio-based products can remain competitive when traditional companies weather economic challenges. We've begun to consider the resilience of our bio-concrete product in light of this insight.
      This information has been instrumental in shaping our strategy and approach as we move forward with our bio-concrete venture.

    High School Regional iGEM Teams Meet Up

    Context:
      This is a regional conference that lies in our fourth stage, in a hope to enhance our product’s implementation capability by sharing and hearing from different products’ creators and promotors, and interact with organizations of different philosophies. We hope to engage different public, and provide a platform for teams to identify similarities, differences, and then collaborate and learn with like-minded friends, and to improve each other’s projects. Hence, this is how the conference was born. The conference incorporated a series of functions, including a controversial biosafety debate, regular presentation, and Q&A sessions.
    High School iGEM Teams Meet Up
    Rationale:
      To achieve the purpose of proposing a chance to engage with different teams from different parts of China, we asked four teams in total to engage in this round table conference. They come from different provinces and regions in China, and we together gathered in Shenzhen, China. Thanks previous friendly and concrete connection established with Link Spider, they were willing to provide us a conference room for us to gather. Hence, our in-person venue was Link Spider’s conference room. For teams and people who couldn’t join the conference in-person, we also provided online streaming options in Tencent Meeting.
    How it is proceeded:
      For the Links team:
    • Presentation: 15-20 min
    • Evaluation by on-site instructors
    • Open Q&A session
      For the Basis team: Same
      For the SCIE team: Same
      For the Keystone team: Same
    Biosafety debate
    • Host Introduces the Topic and Sets the Theme (Total Duration: 10 minutes)
    • Discussion Speaking Segment: Each team may sequentially present their views based on the questions posed by the host and offer rebuttals or further elaborations on the points made. (Note: We aim to create a relaxed and open discussion environment without rigid speaking rules. We encourage active participation, sharing ideas, maintaining silence while others speak, and respecting every team member, judge, and the host.)
    • Each Team Gives Summative Remarks in Order (In alphabetical order of team names: BASIS-China, GreatBay-SCIE, Keystone, LINKS-China) (Total Duration: 30-45 minutes)

    Second Interview with Links Spider

    Company's Website & CEO
    Context & Rationale:
      Team Keystone has successfully developed a friendly connection with the founders of Link Spider since our initial online interview, where we discussed the future prospects of biotechnology and sought advice for emerging biotech startups. However, following this interview, some of our team members and advisors began to express concerns about our lack of knowledge regarding government agencies' attitudes towards promoting biobased construction materials. We were particularly interested in understanding the level of support typically offered by these agencies in our sector to assess the potential and feasibility of our product. We also wanted to determine whether this support predominantly came from government sources. What bothered us was our apparent lack of experience and connections with these agencies. Interestingly, after a High School Regional Teams Meetup, where we had the chance to use a conference room at Link Spider, one of our team members saw an opportunity to address these concerns directly with Link Spider's founders in person. It seemed both convenient and timely, given our proximity to their company at the time. This informal and straightforward approach allowed us to politely inquire about their perspectives on this matter. Consequently, this is how a relaxed yet informative second interview came to be.
    Lessons We Learned:
    • Market-Driven Growth: In the current landscape, the impetus for growth in the biotech and bioindustrial sector in China primarily stems from market dynamics rather than substantial government support. The prevailing trend indicates that market incentives outweigh government attention. Therefore, it may not be crucial to seek government consultation in this context.
    • Industry-Wide Support: Government support, when available, typically targets the entire industry rather than individual companies. In our case, Link Spider has not received significant government support, so our primary sources of support and growth opportunities come from market-driven factors.
    • Diverse Customer Base: There are typically two key customer segments with needs in this sector: individuals or entities beyond the basic sustenance level, such as the Z-generation, and established companies, including construction firms. Many companies are leveraging these needs as a new selling point, especially in the case of well-established construction companies. They are now pursuing initiatives that extend beyond mere profit generation and are aligned with higher standards and sustainability.


    ⛑️ Hardware Constraints:

    Troubleshooting with a modeling expert


    Online Meeting in Tencent
    Context:
      As we near completion of our modeling work, it's crucial to ensure its accuracy and validity. We scheduled an online session with Mr. Su for September 1st, during which he meticulously reviewed our models and provided valuable insights.Modeling is integral to our project, helping visualize processes and calculate crucial experimental data. A professional review is essential to guarantee the precision and validity of our models and to identify areas for improvement.Having previously engaged in an online course with Mr. Su in August, our modeling group reached out to him again, seeking his expertise to review our work.
    What We Learned:
      Mr. Su conducted a detailed review, offering valuable advice. He pinpointed issues such as using an incorrect function to model cyanobacteria growth and recommended suitable models for related variables. These specific suggestions directly improved the quality of our models.
      He also emphasized the importance of not creating models just for the sake of it. Each model should serve a distinct purpose, whether visualizing a biological pathway or calculating a specific variable. This reminder prompted us to reflect on our previous models and maintain a clear connection between our models and the experiment's goals.


    🧬 Engineering Design Constraints

    Dr. Roberson from Johns Hopkins University

    Dr.Robertson
    Context:
      In addressing the four constraints of our project during the fourth stage of Human Practices, the technological constraint, specifically related to the biological engineering aspect, demands professional biological expertise. Our approach involved conducting a face-to-face interview with Dr. Roberson from Johns Hopkins University, which is detailed in the full-length interview description here. A face-to-face interview with an expert offers the most effective platform for comprehensive discussions about our project's engineering and technology. Compared to virtual interviews, physical meetings with college-level professors enable in-depth responses and foster stronger connections between interviewer and interviewee, aligning with the goals of Human Practices activities.
      Dr. Christov Roberson, a biological sciences lecturer at Johns Hopkins University, has experience with iGEM and was previously involved with the Harvard team a decade ago. Our team leader, having been his pre-college student, introduced our project idea. Following initial conversations, a formal in-person interview took place with supporting materials. Learn more about Dr. Roberson here.
    Key Insights
      We should be mindful of potential environmental impacts our chassis organism, Synechocystis PCC 6803 (a cyanobacteria), could trigger, such as freshwater system blooms. Implementing a programmed apoptosis system can mitigate this risk.
      The final product must undergo testing in real-world, outdoor conditions to address constraints beyond theoretical and lab settings.
      Beyond the product itself, it is beneficial to conduct multiple biology-related tests, including assays of enzymatic activity and enzyme location.

    Dr. Bednarcyzk from University of Chicago

    Dr.Bednarcyzk
    Context:
      While our project is making continuous progress, we recognize the need for clarity and details to ensure our final product's safety, eco-friendliness, and applicability. Our project's vision remains a biomaterial serving as a building block for construction and a repair material for existing structures.
      Our concerns primarily revolve around bioengineering aspects, with real-life applications not yet in focus. However, this interview served as a reminder to maintain a comprehensive view of our project.
      For credible feedback from an expert, an interview was the best approach. It allowed us to present our BioBrick designs comprehensively and engage in detailed discussions, enhancing the effectiveness of receiving feedback.
      We reached out to Dr. Robert Bednarcyzk, an associate professor at the University of Chicago's Biological Sciences Learning Center. Dr. Bednarcyzk's biology expertise and teaching experience made him a valuable source of insightful suggestions.
    Key Takeaways
      Dr. Bednarcyzk emphasized the importance of structural integrity within our project, prompting us to consider not only the bioengineering aspect but also the material's integrity. This feedback led us to explore the development of reliable bricks to contain bacteria and algae.
      He suggested focusing on repairing cracks in existing constructions as a safer application for our newly developed material, shifting our project's focus from bricks to crack repair.
      Dr. Bednarcyzk raised concerns about the feasibility of maintaining bacteria's viability within our product. He reminded us to consider nutrition, byproducts of bacteria and algae metabolism, and the remains of microorganisms. These considerations prompted us to reevaluate the viability of our project's design.

    Dr. Frank from Columbia University

    Dr.Frank
    Context:
      On July 26, 2023, we had a discussion with a Columbia University Professor as part of our project's experimental phase. This conversation aimed to provide support for the project's further development.
    Rationale:
      The discussion encompassed various potential challenges we may encounter in the future. By engaging with our team members and leader, we aim to address these questions as our research progresses. Reflecting on these inquiries has enhanced our project's comprehensiveness and our understanding of its scope.
      Dr. Frank, a professor at Columbia University's summer school program, where one of our team members attended, specializes in modern biology and possesses extensive knowledge of genetic engineering.
      After carefully reviewing our project materials, Dr. Frank raised several important questions that warrant consideration in real-life application. Many of these issues have already been addressed by our team:
    • The structural integrity of the biomaterial and its ability to support a building.
    • Strategies for maintaining the viability of cyanobacteria and addressing potential odor concerns.
    • Differentiating our biomaterial from conventional filters or air conditioning systems.
    • Exploring the practicality of using the biomaterial for repairing broken bricks after construction.

    Second Interview with Dr. Chen

      We have maintained friendly relationship with Dr. Chen since our first interview in the conceptualization stage. As our engineering principles grows we seek to find guidance from this old mentor again. This interview has provided us useful information regarding to biosafety, experiment design, public attitude, and publication strategy.
    Context:
      As our project advances, involving numerous experiments, new ideas, and challenges have arisen. These encompass biosafety, bacterial activity, and publication strategies for our bio-brick product. Understanding public attitudes toward our project and assessing our publication approach have become crucial.
      To address these evolving needs, we conducted an interview with Dr. Chen from Tianjin University, an expert in synthetic biology and cyanobacteria. Dr. Chen's expertise provides valuable insights and guidance for our project's future.
    Key Takeaways
      Dr. Chen highlighted the potential for cyanobacteria release into water bodies, possibly leading to eutrophication. Considering the limited availability of suitable suicidal triggers for cyanobacteria, we're exploring additional research in this area.
      Dr. Chen supported our idea of mixing culture medium into hydrogel to sustain cyanobacteria, with sunlight exposure for about 10 minutes daily. This confidence boost encourages us to continue with our current method.
      The use of hydrogel for bacterial encapsulation raised no safety concerns according to Dr. Chen, as it's widely used in research. Sodium alginate emerged as the preferred hydrogel for cyanobacteria encapsulation, a vital piece of information for our bio-brick development.
      Dr. Chen emphasized the role of public perception influenced by commercial advertisements regarding GMOs. Public education and project promotion, especially in synthetic biology, are critical for acceptance.
      For brick-making hardware, Dr. Chen advised starting with smaller bricks and gradually scaling up. This approach aligns with our idea of conducting pre-experiments with small bricks, eliminating ineffective methods, and then progressing to larger sizes. Learning from both failures and successes is key to reaching our final product.

    🏆 Closing the Loop

    Third interview with Dr.Chen

      As we approach the culmination of our project, having successfully completed the initial phases and arriving at the end of the fourth Implementation HP stage, we turn our attention to receiving feedback and comprehensively evaluating our product's impact on the world.
      To close the loop effectively and gather valuable insights, we engaged in an interview with a prominent cyanobacteria researcher. This conversation allowed us to systematically receive feedback on our project's progress and assess its real-world implications. It served as a critical step in ensuring that our product aligns with our initial goals and positively influences the world.
      Dr. Chen's expertise in cyanobacteria-related projects and general HP and PE initiatives made him an invaluable source of feedback and guidance as we enter this final stage of our project.
      We conducted this HP experience by re-engaging in an interview with Dr. Chen, emphasizing our commitment to closing the loop and evaluating our product's impact on the world.
    Key Takeaways
      Chen underscored the importance of addressing real-world problems in our HP and PE projects, emphasizing the need to make our solutions attainable. While we had already established the significance of solving carbon dioxide emissions from traditional concrete in our HP cycle, his insights inspired us to further refine our product's real-world impact.
      He also recommended addressing the prevalent bias associated with cyanobacteria as potential causes of algal blooms in PE projects. We took this advice seriously and executed several PE initiatives related to cyanobacteria, including creating a social platform article, a coloring book, and delivering a lecture at our school. These actions align with our commercialization goals by improving public perception and engagement.

    Evaluaion of the Market Potential of BioBrick

      As we complete the development of a functional BioBrick product which is produced by incorporating engineered cyanobacteria in a hydrogel-sand matrix and can act as both a sealant for improving compressive strength of concrete and a brick for acting as construction material, it is necessary to evaluate the place of our innovative product in the construction materials market. A comprehensive analysis using the SWOT (strengths, weaknesses, opportunities, and threats) evaluation matrix—a commonly accepted model for identifying factors that affect future operations for the business of a specific product and for setting strategic goals—is made for BioBrick to provide an overview for the potentials and challenges for this product in the construction materials market. From the evaluation matrix, it can be seen that our product has unique strengths and limited weakness, which gives it several opportunities and certain challenges if introduced to the market.

    Strengths Weaknesses Opportunities Threats
    1. Reduces atmospheric carbon dioxide emissions via intake of the gas by engineered cyanobacteria incorporated in the material and preventing emissions (which would be the case in production of traditional concrete), thus achieving sustainable construction.
    2. Improves compressive strength and increases age of concrete when applied for crack-repair due to the formation of calcium carbonate precipitates.
    3. Has dual-functionality of acting as both a sealant and a brick.
    4. Simple method of production without tremendous heat input.
    1. The per-unit production cost is higher than traditional cement and concrete.
    2. Potential disapproval by consumers towards an unfamiliar product.
    3. No currently established production line, leading to low total production capacity.
    1. New product introduced to the traditional construction materials market.
    2. Absence of any Bioconcrete company in the local context of China.
    3. Strong support from the government oriented to biotechnology.
    4. Companies aim to reduce the amount of carbon taxation by introducing sustainable technologies to reduce burden on business.
    5. Widespread application of cement and concrete in architecture provide large population of consumers.
    1. Current production price of concrete is lower than that of our product, making traditional concrete more favorable to consumers.
    2. The traditional construction materials market in China is already saturated with large government-owned institutions and international corporations.
      Maximize opportunities with strengths:
    1. The unique benefit of sustainability of our product should be emphasized through marketing campaigns.
    2. We should perform campaigns to reach out to corporations that are seeking for sustainable substitutes. An example is the ZhenYe real estate company which we contacted.
    3. We can easily increase production with aid of government support due to the simplicity of the method of production.

      Overcome weakness to retain opportunities:
    1. To eliminate the concerns from the public regarding the functionality and safety of our innovative product, we should perform public education events that are offered to both the general public (the end-users) and construction companies. The public education events can be extensions to events we have delivered this year.
    2. We should perform campaigns to reach out to corporations that are seeking for sustainable substitutes. An example is the ZhenYe real estate company which we contacted.
    3. We should try to seek government subsidy to decrease the relatively high production cost.

      Minimize threats with strengths:
    1. The heavy carbon taxation imposed by the government drives businesses to reach net zero carbon emissions to decrease their operational cost. By implementing our product, construction companies can largely reduce the amount of taxation they have to pay, making our product favorable to companies.
    2. Our product can act as a better substitute for traditional concrete/cement functionality-wise since it retains the dual-functions of the traditional material while allowing for carbon dioxide intake. This unique strength allows us to enter both the cement and concrete market and be the pioneer as a biomaterials business in these markets, therefore having unique competitive strength.

      Overcome weakness to prevent threats:
    1. Minimize production cost by further investigating the minimum amount of each raw material involved in production while retaining compressive strength similar to Portland cement.
    2. At the beginning of the business, specifically target international corporations as the main consumers. This is because these companies are more likely to seek for sustainable materials since they would likely have spare capital to spend on the additional cost of new materials and would wish to establish an environmentally friendly image for themselves, as in the case of Cargill.


    To conclude, the unique strength of sustainbility of our product can make it a competitive substitute to traditional concrete/cement and its cost is similar to the traditional materials since it reduces carbon taxation for corporations. In the future, we will develop a more mature method for the large-scale production of BioStone and perform more Public Education campaigns to build a firm foundation for starting as a business.