Team

General Tasks Overview

	PI	Advisor				Wet Lab								Dry Lab						Human Practice
	Wenwen Huang	Kexin Nie	Jingyi Tian	HUONG THAO DOAN	Yilan Cui	Ximeng Zheng	Shihan Xiong	Jingyi Zuo	Weilun Du	Minjie Kuang	Min Yuan	Yidan Xu	Ruijie Ding	Yifan Deng	Xinlu Zhu	Yu Bai	Yitian Xiao	Yao Sun	Mingyuan Wang	Yiyang Zhang	Haiyun Kong	Xiang Yao	Di Wu	Kexuan Kong
Analysis				●					●	●											●
Conceptualization	●	●				●	●	●	●	●		●		●	●	●	●			●	●	●	●	●
Background Research						●	●	●	●	●	●	●	●	●		●				●	●	●	●	●
Investigation	●	●				●	●	●	●	●	●	●	●	●	●	●	●			●	●	●	●	●
Notebook/record keeping								●	●	●
Project Administration						●	●	●	●					●				●		●
Fundraising						●
Public Engagement																				●	●	●	●
Hardware								●			●
Wiki Coding																		●	●
Visualization					●	●	●	●			●			●	●	●	●	●		●	●	●		●
Writing						●	●	●	●	●	●	●	●	●	●	●	●		●	●	●	●	●
Safety			●				●					●	●								●
Other	●	●	●				●												●

Attribution Form

Name	Role	Tasks	Specific Tasks
iGEM Competition 2023: Attribution Form
Team: 2023 iZJU-China
Team Member Contributions
Wenwen Huang	PI	Conceptualization, Investigation, Other	She provided help for team members to brainstorm and gave useful suggestions; She provided general advice on the experiments when we faced setbacks; She interacted with professors in related fields to provide experimental support to team members.
Jingyi Tian	Advisor	Safety, Other	She kept the lab regulations informed by all student members; she ordered and supplyed reagent consumables needed.
Kexin Nie	Advisor	Conceptualization, Investigation, Other	She helped student members come up with the initial idea for the project, provided suggestions on troubleshooting; she trained student members in experimental skills and guided them to conduct experiments properly; she supervised all the work that the iGEM team had done and ensured that they operate in accordance with test specifications and obey the laboratory safety rules.
HUONG THAO DOAN	Advisor	Analysis; Investigation	She analyzed data generated by the turpentine user survey; she made the turpentine user survey.
Yilan Cui	Advisor	Visualization	She was responsible for the promotion video production.
Ximeng Zheng	Student Leader	Conceptualization, Background Research, Investigation, Project Administration, Visualization, Writing, Fundraising	she participated in brainstorming and developing initial ideas for the project; she read peer-reviewed scientific research articles to build initial experimental proposals and protocols; she performed the plasmid transformation, test of biphase reaction system; she managed and coordinated the whole project; she prepared the promotion and presentation videos; she wrote the content of "engineering" part for wiki, reviewed and editedg content for the wiki; she raised funds and in-kind donations from our university to support the project.
Shihan Xiong	Student Leader	Conceptualization, Background Research, Investigation, Project Administration, Visualization, Writing, Safety, Other	She participated in brainstorming and developing initial ideas for the project, also brainstorming team name and slogan; she read peer-reviewed scientific research articles to build initial experimental proposals and protocols; she performed experiments including protein purification and SDS-PAGE; she managed and coordinated the whole project as well as planning and executing the safety experiments; she prepared the presentation video; she wrote the contents of "safety" section for wiki, Biosafety Handbook and safety form, as well as reviewed and edited other content for Wiki; she reviewed the safety guidelines of the institution and government, organized team members to review laboratory safety and managed experiments related to project safety; she chose and ordered reagent consumables needed.
Yifan Deng	Student Leader	Conceptualization, Investigation, Visualization, Writing, Project Administration, Background Research	He participated in brainstorming and developing initial ideas for the project, also brainstorming of the modeling and wiki project; he used Simbiology to establish alpha-pinene oxide degradation part of the reaction model and modify the parameters; he used Simbiology to visualize the reaction process and participated in preparation for promotion video; he wrote the content of "modeling" part for wiki; he managed and coordinated the dry lab and participated in decision making on some major team issues; he read scientific articles related to alpha-pinene oxide degradation and model construction.
Yiyang Zhang	Student Leader	Conceptualization, Background Research, Project Administration, Public Engagement, Visualization, Writing, Investigation	She participated in brainstorming and developing initial ideas and product design for the project; she learned the Wiki of previous iGEM teams to get insight into how the Human Practice work was conducted; she read the iGEM handbook and sort out the requirements of the iGEM competition; she searched some initial literature to help with developing initial ideas; she managed and coordinated the whole human practice part of the project, including activities, planning, and execution; she collaborated and communicated with volunteer teaching teams, 0799 Art District, and different stakeholders to engage the broader community; she prepared the promotion and presentation videos; she wrote some parts of Human Practice and Team for wiki, reviewed and editedg content for the human practice part of wiki; she collected the turpentine user survey, the offline questionnaire and the online survey.
Jingyi Zuo	Student Leader	Conceptualization, Background Research, Investigation, Notebook/record keeping, Project Administration, Hardware, Visualization, Writing	She particapted in brainstorming and developing ideas for the project, including the original inspiration for the project and three initial proposals; she read peer-reviewed scientific research articles to build initial experimental proposals and protocols; she performed experiments including the plasmid transformation, protein purification, SDS-page, plasmid extraction, test of biphase reaction system, and primer design; she maintained the laboratory notebook and kept a record of partial laboratory activities; she managed and coordinated the project activities of initial plan; she designed our product and its mechanical composition; she prepared the promotion and presentation videos; she wrote, revied and edited content for the Wiki, including experimental protocol and minutes of meeting.
Weilun Du	Student Leader	Analysis, Conceptualization, Background Research, Investigation, Project Administration, Notebook/record keeping, Writing	She participated in data analysis for the qualitative analysis report plot and TIC plot of the GC-MS test; she collected the literature, participated in the brainstorming of the whole project and specific experimental process, also participated in the troubleshooting and scheme optimization; she read peer-reviewed scientific research articles to build initial experimental proposals and protocols; she performed experiments including bacteria transformation, protein purification and construction of biphase reaction system; she managed and coordinated the wet lab and participated in decision making on some major team issues; she recorded the experimental progress and results in stages, reported experimental progress to our PI; she wrote the experimental protocols presented on the Wiki, collected data and wrote the outline for parts.
Minjie Kuang	Student	Analysis, Conceptualization, Background Research, Investigation, Notebook/record keeping, Writing	She participated in data analysis for the qualitative analysis report plot and TIC plot of the GC-MS test; she participated in brainstorming before the initiation of experiments to determine the protocol and whole process, also developed ideas for experiment improvement; she read peer-reviewed scientific research articles to build initial experimental proposals and protocols; she performed experiments including bacteria transformation, protein purification, SDS-PAGE with Coomassie Brilliant Blue staining for protein validation, construction of biphase reaction system; she maintained the laboratory notebook, and kepy a record of all laboratory activities with simple analysis and troubleshooting; she integrated all data obtained in the wet lab, and wrote words for wiki "lab notebook" and "parts" section.
Yao Sun	Student	Wiki Coding, Visualization, Project Administration	She is one of the main programmers for the team wiki, mainly taking charge of the homepage and all the content pages; she designed some parts of the wiki; she managed and coordinated the wiki group.
Xinlu Zhu	Student	Conceptualization, Investigation, Visualization, Writing	She participated in the brainstorming of the modeling project; she used Simbiology to establish the reaction model and modify the parameters; she used Simbiology to visualize the reaction process; she wrote the content of "model" part for wiki.
Xiang Yao	Student	Conceptualization, Background Research, Public Engagement, Investigation, Visualization, Writing	She helped with coming up with the initial ideas for the project; she read several peer-reviewed scientific research articles for thorough background research; she learned the Wiki of previous iGEM teams to get insight into how the Human Practice work was conducted; she searched some initial literature to help with developing initial ideas; she conducted interviews with some oil painting artists, she visited some oil paint stores to conduct the market survey; she collected the offline questionnaire; she participated in video production, preparing the video scripts; she wrote, reviewed, and edited content for the Wiki, she wrote content for WeChat tweets and Instagram.
Min Yuan	Student	Investigation, Background Research, Handware, Visualization, Writing	She performed experiments including bacteria transformation and SDS-PAGE for protein validation, and performed Gibson assembly including PCR test, DNA gel running and bacteria transformation; she read peer-reviewed scientific research articles to build initial experimental proposals and protocols; she used computational method to create a draft version of our model; she prepared the promotion video; she wrote words for wiki "design" section and produced flowchart through BioRender, integrated words for wiki "results" section.
Kexuan Kong	Student	Conceptualization, Background Research, Visualization, Investigation	She participated in brainstorming and developing ideas for the project; she learned the Wiki of previous iGEM teams to get insight into how the Human Practice work was conducted; she was responsible for the art design of the wiki web pages and produced animations for the video; she collected the offline questionnaire.
Yidan Xu	Student	Conceptualization, Background Research, Investigation, Writing, Safety	She participated in the brainstorming of the whole project; she read peer-reviewed scientific research articles to build initial experimental proposals and protocols; she performed preliminary experiments and Gibson assembly; she wrote the content of "implementation" part for wiki and Biosafety Handbook; she checked the Safety Form and reviewed the safety guidelines of the institution.
Haiyun Kong	Student	Analysis, Conceptualization, Background Research, Public engagement, Writing, Safety, Visualization, Investigation	She analyzed the data of surveys in human practice; she helped us come up with the initial idea for the project; she learned the Wiki of previous iGEM teams to get insight into how the Human Practice work was conducted; she cooperated with volunteer teaching teams and collected questionnaires from the public; she wrote some parts in the human practice column in the wiki; she worked with wet lab members to discuss which safety problems need to be considered; she was in charge of video production, collecting materials needed for video; she collected the turpentine user survey, the offline questionnaire and the online survey.
Yitian Xiao	Student	Conceptualization, Investigation, Visualization, Writing	He participated in the brainstorming of the modeling project; he used Simbiology to establish the reaction model and modify the parameters; he used Simbiology to visualize the reaction process; he wrote the content of "modeling" part for wiki.
Ruijie Ding	Student	Investigation, Background Research, Writing, Safety	She performed experiments including protein purification, Gibson assembly and Kirby-Bauer test; she read peer-reviewed scientific research articles to build initial experimental proposals and protocols; she wrote the content of "proof-of-concept" part for wiki; she participated in writing protocol of safety experiment.
Yu Bai	Student	Conceptualization, Investigation, Visualizations, Writing, Background Research	He participated in brainstorming before the initiation of experiments to determine the protocol and whole process. He also proposed a combing expression system of pra-pol and P450 engineering bacteria; for dry lab, he designed the comprehensive models and completed plasmid construction plans; for wet lab, he performed SDS-PAGE and spectrophotometry; he used Simbiology to visualize the reaction process; he integrated the data obtained in the comprehensive model, write for wiki "Modeling" part; he read scientific articles related to alpha-pinene oxide degradation and model construction.
Di Wu	Student	Conceptualization, Background Research, Public Engagement, Writing, Investigation	She participated in brainstorming and developing ideas for the project; she read peer-reviewed scientific research articles online about turpentine to help understand the background of the project; she learned the Wiki of previous iGEM teams to get insight into how the Human Practice work was conducted; she connected with a volunteering teaching team, participated in, and organized activities about synthetic biology; handed out questionnaires to different groups; she wrote the HP overview. Joined in the writing part of communication, education, and collaboration. Produce and edit tweets to advertise our teams; she collected the offline questionnaire.
Mingyuan Wang	Student	Wiki Coding, Writing, Other	He is one of the main programmer for the team wiki, mainly take charge of homepage, menu and member page; he wrote and modified some parts of the wiki; he supply bilibili account for official advertise.

Name	Institution Type	Relationship To Team	Tasks	Specific Tasks
External Contributions
Asimov	Industry	Sponsor	Software	Provided a software that allows precise design, simulation, and visualization of plasmid assembly and cloning procedures for this project.
IDT	Industry	Sponsor	Software	Provided a software that allows precise design and optimization of the primers.
ChatGPT	Industry	Contractor	Writing	We used ChatGPT-3.5 for the initial draft of writing the "Chapter 5 Safety literacy" of Safety Handbook. The content was verified and re-write by the team. We also learned some of the code ideas from ChatGPT - 3.5.
Sangon Biotech (Shanghai) Co.,Ltd	Industry	Contractor	Comceptualization, investigation	(1) Conceptualization - Provided valuable suggestions for plasmid construction and insertion site selection of target genes; (2) Investigation - Provided whole gene synthesis, plasmid assembly and primer synthesis services to the team.
BioRender	Industry	Contractor	Software	We used BioRender to create the work including workflow graphics and other schematic diagram on the wiki.
Snapgene	Industry	Contractor	Software	Provided a software that allows precise design and optimization of the primers.
Department of Chemistry, Zhejiang University	Academic	Collaborator	Investigation	Performed the GC-MS tests for this project.
Yi Xiong	Academic	Collaborator	Project Administration	A PhD student in our university, provided us with a plasmid (PET-28a).
Mengdi Jin	Academic	Collaborator	Project Administration	The core facility technician assists us in comunicating with the college and get help from the college.
Quan Yang	Academic	External Advisor	Project Administration	A PhD student in our university, assisted in conducting electroporation.
Wenguang Wang	Academic	External Advisor	Project Administration	A PhD student in our university, assisted in conducting electroporation.
Di Chen	Academic	External Advisor	Project Administration	He is a professor of our institute who gave us some guidance on the wet experiment.
Lisheng Kong	Academic	External Advisor	Wiki Coding	He helped solve some of the difficulties encountered in writing wikis
Jinkun Xiao	Academic	External Advisor	Wiki Coding	He helped to write some wikis
Xiangdong Art Museum (0799 Art Zone)	Government	Collaborator	Other	It provided services requested by the team including the human practice part and the product design.
Xun He	Other	External Advisor	Other	He is an artists and provided us with suggestions on product form.
Jianyi Geng	Other	External Advisor	Other	He is an artist and provided us with background information.
Baiqiao Zhang	Other	External Advisor	Other	He is an artists and provided us with information about turpentine and oil painting.
Jue Hu	Other	External Advisor	Other	He is an artist and provided us with suggestions for product positioning.
Donghua Ouyang	Other	External Advisor	Other	She is an artists and provided us with information of turpentine.
Xiangmin Zhu	Other	External Advisor	Other	He is an artist and provided us with background information.
Oil paint stores	Other	External Advisor	Other	They provided some market information.
International Campus Zhejiang University - Juxi Cangnan Wenzhou Summer Social Practice Group	Other	Collaborator	Public Engagement	It prepared a volunteer teaching activity to engage the broader community.
"Haishishanmeng" Volunteering Teaching Team of Zhejiang University	Other	Collaborator	Public Engagement	It prepared a volunteer teaching activity to engage the broader community.

Project Timeline
Project Activity	Start Date	End Date	Total Time (weeks)
1. Recruit Team Members	2023/01/06	2023/06/08	22
2. Development of Project Plan	2023/02/15	2023/04/24	10
3. Wet Laboratory Activities	2023/08/06	2023/10/10	10
4. Dry Laboratory Activities	2023/06/24	2023/10/10	15
5. Building the Team Wiki	2023/06/20	2023/10/12	16

ATTRIBUTIONS

General Tasks Overview

Attribution Form