New Parts

Name	Type	Short Description	Designer	Length (bp)
BBa_K4807000	Coding	hemA(s) Gene	Vaios Styliaras	1257
BBa_K4807001	Regulatory	T7 Promoter + RBS Window from pETDuet-1	Vaios Styliaras	120
BBa_K4807002	Terminator	T7 terminator from pETDuet-11	Vaios Styliaras	86
BBa_K4807003	Coding	CAT gene	Vaios Styliaras	1584
BBa_K4807029	RBS	RBS from pETDuet-1	Vaios Styliaras	43

Existing Parts

Name	Type	Short Description	Designer	Length (bp)	Group
BBa_K3630004	Tag	S-tag	Leong Chan Tat	45	iGEM20_UM_Macau
BBa_K1150016	Tag	HA-tag	Freiburg 2013	27	iGEM13_Freiburg
BBa_K3033006	Tag	6-His Tag	Philip Naderev P.Lagniton	18	iGEM19_UM_Macau
BBa_K3071017	Tag	Myc Tag	Leung Hei Man	36	iGEM20_UM_Macau
BBa_K4875014	Coding	Rluc8, a mutated variant of a luciferase from Renilla Reniformis with an absorbance maximum at around 410nm.	Cheng Ziyan	936	iGEM23_Metropolis-Shanghai
BBa_I716155	Coding	hemD gene, encoding HemD or uroporphyrinogen III synthase, involved in the heme biosynthesis pathway and an upstream regulator for 5-ALA accumulation.	Kristin Doan	741	iGEM07_Berkeley_UC
BBa_K2779006	Coding	hemF gene encoding O2 dependent coproporphyrinogen-III oxidase; EC 1.3.3.3;. It is responsible for catalyzing Coproporphyrinogen-III production in the heme biosynthesis pathway and a downstream regulator for 5-ALA accumulation.	than Agena, Rochelin Dalangin, Anna Kim, Irene Shkolnikov	900	iGEM18_UAlberta
BBa_K1977000	Coding	rhtA gene encoding a membrane protein that is involved in the efflux of threonine and homoserine. Can also export other amino acids such as proline, serine, histidine, cysteine and aminolevulinic acid.	Camila Gaspar	967	iGEM16_Westminster_UoW
BBa_K4759025	Coding	hemL gene encoding glutamyl-tRNA reductase (GluTR), which catalyzes the first step of porphyrin biosynthesis.	CMengrui Tao	1281	iGEM23_Jiangnan-China

Name	Type	Short Description	Designer	Length (bp)
BBa_K4807021	Composite	hemD insert	Vaios Styliaras, Efi Chatzopoulou	992
BBa_K4807022	Composite	CAT insert	Vaios Styliaras, Efi Chatzopoulou	1629
BBa_K4807023	Composite	rhtA insert	Vaios Styliaras, Efi Chatzopoulou	1053
BBa_K4807025	Composite	hemF insert	Vaios Styliaras, Efi Chatzopoulou	1056
BBa_K4807026	Composite	hemA(s) insert	Vaios Styliaras, Efi Chatzopoulou	1413
BBa_K4807027	Composite	Rluc8 insert	Vaios Styliaras, Efi Chatzopoulou	1142

Name	Type	Short Description	Designer	Length (bp)
BBa_K4807004	Primer	Forward Primer for hemD insert	Vaios Styliaras	35
BBa_K4807005	Primer	Reverse Primer for hemD insert	Vaios Styliaras	35
BBa_K4807007	Primer	Forward Primer for CAT insert	Vaios Styliaras	35
BBa_K4807008	Primer	Reverse Primer for CAT insert	Vaios Styliaras	35
BBa_K4807009	Primer	Forward Primer for rhtA insert	Vaios Styliaras	35
BBa_K48070010	Primer	Reverse Primer for rhtA insert	Vaios Styliaras	35
BBa_K48070011	Primer	Forward Primer for hemL insert	Vaios Styliaras	35
BBa_K4807012	Primer	Reverse Primer for hemL insert	Vaios Styliaras	35
BBa_K4807013	Primer	Forward Primer for hemA(s) insert	Vaios Styliaras	35
BBa_K4807014	Primer	Reverse Primer for hemA(s) insert	Vaios Styliaras	35
BBa_K4807015	Primer	Forward Primer for hemF insert	Vaios Styliaras	35
BBa_K4807016	Primer	Reverse Primer for hemF insert	Vaios Styliaras	35
BBa_K4807017	Primer	Forward Primer for Rluc8 insert	Vaios Styliaras	35
BBa_K4807018	Primer	Reverse Primer for Rluc8 insert	Vaios Styliaras	35

Name	Description	Origin	Length (bp)
BBa_J435116	pOSIP-TT (TetR, P21)	(FreeGenes)	7220
BBa_K4807019	pET-29c(+)	(addgene)	5372
BBa_K4294093	pTU2-A-RFP	Moore et al, Paul Freemont lab	3286
BBa_J100519	pETDuet-1	Campbell_M_Lab	5420