Team Member Contributions

Name	Role	General Tasks	Specific Tasks
EFTERPI CHATZOPOULOU	Student Leader	Analysis, Conceptualization, Background Research, Investigation, Notebook/record keeping, Project Administration, Fundraising, Public Engagement, Writing, Safety, Other	1. Analysis - She analyzed data from literature and other iGEM projects in order to design our project. 2. Conceptualization - She participated in the brainstorming sessions of our team and the development of our project idea. 3. Background Research - She conducted thorough research which involved studying relevant literature and collecting data essential for our project idea and design. 4. Investigation - She took part in both the wet lab planning and experiments. 5. Notebook/record keeping - She was responsible for maintaining the lab notebook and keeping track of our experiment results. 6. Project Administration - As our team leader, she organized our team activities and arranged the roles and tasks for all team members. She consistently reviewed the progress made by other team members and offered advice. 7. Fundraising - She reached out to the University of Ioannina and the Regional Fund of Epirus, successfully negotiating an agreement for covering our competition expenses. 8. Public Engagement - She participated in all of our team's human practices and science communication actions, including conferences, collaborations with other iGEM teams and integrated human practices. 9. Writing - She wrote many texts incuding e-mails, human practices and wet lab documents, social media and wiki content. 10. Safety - She received basic safety training from our instructor and PI and was responsible for filling in the team's safety form with the other wet lab members. 11. Other - She was the head of Social Media team.
Vaios Styliaras	Student	Analysis, Conceptualization, Background Research, Fundraising, Public Engagement, Visualization, Writing, Safety	1. Analysis - He analyzed data from literature and other iGEM projects in order to design our project. 2. Conceptualization - He participated in the brainstorming sessions of our team and the development of our project idea. 3. Background Research - He conducted thorough research which involved studying relevant literature and collecting data essential for our project idea and design. 4. Fundraising - He also reached out to the University of Ioannina, successfully negotiating an agreement for covering our team registration expenses. 5. Public Engagement - He participated in most of our team's human practices and science communication actions, including conferences, collaborations with other iGEM teams and some integrated human practices. 6. Visualization - He created all the scientific visuals for our wiki page, including construct overviews, various diagrams and enzymatic pathways. 7. Writing - He was the person responsible for all the scientific texts of our wiki. He reviewed everything that was going to be incorporated in our website and wrote most of the texts. He also helped with the writing of other documents, such as human practices documents and e-mails. 8. Safety - He was also responsible for filling in the safety form with the other Wet lab members.
Maria- Evryanthi Pantermali	Student	Analysis, Conceptualization, Background Research, Investigation, Notebook/record keeping, Fundraising, Public Engagement, Writing, Safety, Other	1. Analysis - She analyzed data from literature and other iGEM projects in order to design our project. 2. Conceptualization - She participated in the brainstorming sessions of our team and the development of our project idea. 3. Background Research - She conducted thorough research which involved studying relevant literature and collecting data essential for our project idea and design. 4. Investigation - She took part in wet lab experiments. 5. Notebook/record keeping - She also updated the lab notebook and kept track of our experiment results. 6. Fundraising - She reached out to some sponsors in order to secure funding for our team. 7. Public Engagement - Being the head of human practices, she organized and participated in all of our team's human practices and science communication actions, including conferences, collaborations with other iGEM teams and integrated human practices. 8. Writing - She wrote texts incuding e-mails, human practices documents, social media and wiki content. 9. Safety - She received basic safety training from our instructor and PI and was responsible for filling in the team's safety form with the other wet lab members. 10. Other - She was also a member of Social Media team.
Panagiotis Panagopoulos	Student	Analysis, Conceptualization, Background Research, Investigation, Safety	1. Analysis - He analyzed data from literature and other iGEM projects in order to design our project. 2. Conceptualization - He participated in the brainstorming sessions of our team and the development of our project idea. 3. Background Research - He conducted thorough research which involved studying relevant literature and collecting data essential for our project idea and design. 4. Investigation - Ηe participated in few of the wet lab experiments. 5. Safety - He was also responsible for filling in the safety form with the other Wet lab members.
Maria Symeonidou	Student	Analysis, Background Research, Public Engagement, Investigation, Writing, Safety	1. Analysis - She analyzed data from literature and other iGEM projects in order to design our project. 2. Background Research - She conducted research which involved studying relevant literature and collecting data essential for our project idea. 3. Investigation - She took part in wet lab experiments. 4. Public Engagement - She participated in some integrated human practices. 5. Writing - She wrote texts for our wiki page. 6. Safety - She received basic safety training from our instructor and PI and was responsible for filling in the team's safety form with the other wet lab members.
Ioannis Tzitzigiannis	Student	Conceptualization, Fundraising, Writing	1. Conceptualization - He participated in our initial brainstorming sessions. 2. Fundraising - He reached out to multiple sponsors to secure funding for our team. 3. Writing - He wrote e-mails to our sponsors.
Vasileios Vouronikos	Student	Fundraising, Wiki Coding, Visualization, Writing, Conceptualization	1. Conceptualization - He participated in our initial brainstorming sessions. 2. Fundraising - He reached out to multiple sponsors to secure funding for our team. 3. Wiki Coding - He was responsible for building our Wiki website, including coding and layout. 4. Visualization - He took on the role of presenting the team's work on the wiki and managing the user interface. 5. Writing - He wrote e-mails to our sponsors.
Despoina Kissa	Student	Conceptualization, Fundraising, Public Engagement, Visualization	1. Conceptualization - She participated in our initial brainstorming sessions. 2. Fundraising - She reached out to a few sponsors to secure funding for our team. 3. Public Engagement - She participated in a conference and a collaboration with team Patras Med. 4. Visualization - She was responsible for all our graphics, including logo design, social media graphics and general wiki graphics.
Elena Angelopoulou	Student	Public Engagement, Wiki Coding, Writing	1. Public Engagement - She participated in a collaboration with iGEM Thrace. 2. Wiki Coding - She was responsible for building the Wiki website. 3. Writing - She participated in writing some of the wiki content (Home page).
Viktoria Sizikova	Instructor	Background Research, Investigation, Project Administration, Safety, Notebook/record keeping	1. Background Research - She conducted thorough research to determine and arrange the suitable experiments for our project and provide guidance on them. 2. Investigation - She supplied us with all the necessary consumables, reagents and protocols, while also instructing us on how to carry out our experiments. 3. Notebook/record keeping - She guided us on the correct methods for recording our experiment results and maintaining a laboratory notebook. 4. Project Administration - She helped us plan our Wet lab experiments to achieve better results within the given timeframe. 5. Safety - She provided us with the necessary laboratory safety training to ensure that we minimize the potential risks of laboratory accidents.
Theologos Michaelidis	Primary PI	Analysis, Background Research, Investigation, Project Administration, Safety	1. Analysis - He reviewed all of our experimental results and provided guidance on how to proceed with our laboratory work. 2. Background Research - He conducted thorough research to determine and arrange the suitable experiments for our project. 3. Investigation - He provided supervision during our experiments and offered solutions to address any challenges we faced. He also supplied us with all the necessary consumables, reagents and protocols we needed for our experiments. 4. Project Administration - He helped us plan our Wet lab experiments to achieve better results within the given timeframe. 5. Safety - He provided us with the necessary laboratory safety training to ensure that we minimize the potential risks of laboratory accidents.
HARALAMBOS STAMATIS	Primary PI	Conceptualization, Project Administration, Other	1. Conceptualization - He supported us as we explored potential project themes, offering essential advice throughout the process. 2. Project Administration - He guided us on how to organize our project activities, in order to stay on schedule with our experiments and other tasks. 3. Other - He played a mediating role in getting our team registered for the competition, creating the team roster, and ordering our tickets.

External Contributions

Name	Institution Type	Relationship To Team	Tasks	Specific Tasks
Artemis Moustaka	Academic	External Advisor	Investigation	Investigation - She consulted our wet lab experiments when our instructor was temporarily away from the laboratory.
Despoina Mari	Academic	External Advisor	Investigation	Investigation - She also consulted our wet lab experiments when our instructor was temporarily away and helped us with general laboratory preparations (antibiotics, electrophoresis loading buffers, etc.)
Sofia Oikonomou	Academic	Background Research, Safety, Other	Other	Sofia was our mentor in iGEM's Mentorship Program. She played a vital role in reviewing our project and advising us on iGEM's deadlines and requirements. Specifically, she contributed in the following ways: 1. Conceptualization - She helped us develop our project implementation by proposing to us the quorum sensing system. 2. Background Research - She conducted thorough research, including reading relevant papers, and provided us with essential literature related to the quorum sensing system. 3. Safety - She helped us write our safety form and ensuring that we met iGEM's rules and safety regulations. 4. Other - She also showed us how to design our constructs in benchling and provided us with a descriptive list for our necessary laboratory supplies.
Konstantinos Afordakos	Academic	External Advisor	Conceptualization, Other	Konstantinos was iGEM IOANNINA's 2021 team leader. He provided valuable advice to our team, offering a unique perspective as a foreign iGEMer. 1. Conceptualization - He raised many questions regarding the safety and the efficacy of our therapy, which helped us come up with an improved design for IRIS. 2. Other - He also provided guidance on meeting competition deadlines and reaching out to sponsors and professors of our university.
Kyrania Christofi	Academic	External Advisor	Other	Kyrania was the head of wet lab in iGEM IOANNINA 2021. She also consulted our team and through her iGEM experience highlighted the competition's important deadlines and deliverables. Other - She gave us her general advice upon our project and it's implementation, provided us with important information about our wet lab experiments and the reagents that the previous team had left for us to use.
Andreas Vasios	Academic	External Advisor	Fundraising	Andreas was the Fundraising leader of iGEM IOANNINA 2021. Fundraising - He guided our team on how to reach out for sponsors in order to raise the necessary funds for the competition and our project.
iGEM IOANNINA 2021	Academic	External Advisor	Other	Other - The rest of the iGEM IOANNINA 2021 team also helped us by providing advice about our project design, implementation and human practices. They also highlighted the importance of following the iGEM timeline and deadlines.
Eirini Kleidara	Academic	Other	Conceptualization, Background Research, Public Engagement	Eirini is a former iGEM IOANNINA's 2023 member who had to leave the team for personal reasons. During her time in iGEM, she contributed to the team in many different ways. 1. Conceptualization - She participated in our brainstorming sessions, proposing different topics and ideas. 2. Background Research - She did a lot of background research, including reading papers related with our project idea, previous iGEM projects and protocols for our experiments. 3. Public Engagement - She participated in some of our team's human practices and shared our project with the public.
Ioannis Intzes	Academic	Other	Conceptualization, Background Research, Public Engagement	Ioannis is also a former member of team iGEM IOANNINA 2023. He had to leave the team due to personal reasons, but contributed to the team in an important degree. 1. Conceptualization - He also participated in our brainstorming sessions, proposing different topics and ideas. 2. Background Research - He did a lot of background research, including reading papers related with our project idea, previous iGEM projects and protocols for our experiments. 3. Public Engagement - He participated in some of our team's human practices and science communication actions, including conferences.
Charikleia Christana	Academic	Other	Fundraising, Public Engagement	1. Fundraising - She helped us raise funding in order to participate in the competition. 2. Public Engagement - She also contributed to the iGEM Patras Med collaboration for World Health Day, by helping us design our paper sign
Regional Fund of Epirus	Government	Sponsor	Other	The Regional Fund of Epirus sponsored our team and secured our Grand Jamboree team fee, our team member Jamboree tickets and flight and accommodation expenses.
University of Ioannina	Academic	Sponsor	Other	University of Ioannina sponsored our team, securing our Registration Fees.
Biomedical Research Institute - FORTH	Academic	Sponsor	Other	Biomedical Research Institute - FORTH sponsored our team by providing us with a team member ticket for the Grand Jamboree.
METROLAB P. KAPETIS S.A.	Other	Sponsor	Other	METROLAB P. KAPETIS S.A. provided a sponsorship to our team, allowing us to use their support as needed.
Bio-chem Ioannina	Other	Sponsor	Other	Bio-chem Ioannina provided us with a consumables sponsorship from their company.
iGEM Athens 2023	Academic	Other	Other	iGEM Athens 2023 provided us with a crucial plasmid for our wet lab experiments, pTU2-A-RFP (p15A origin).
George Thyphroniti's laboratory - Department of Biological Applications and Technology, University of Ioannina	Academic	Other	Other	Other - They offered us the laboratory space for some of our experiments and most of the required consumables to carry them out.
Sotiris Hadjikakou's laboratory - Department of Chemistry, University of Ioannina	Academic	Other	Other	Other - They supplied us with tetracycline in order to conduct our experiments.
Spyros Petrakis, Ioannis Gkekas - Institute of Applied Biosciences, CERTH	Academic	Other	Other	Other - They provided us with pBBR1MCS-3 bacterial stocks and DB3.1 cell stocks.
Marina Koukaki, Androniki Kretsovali, Τakis Makatounakis - Iannis Talianidis's laboratory, Institute of Molecular Biology & Biotechnology, FORTH	Academic	Other	Other	Other - They supplied us with pETDuet-1, one of the basic vectors for our experiments.

Project Timeline

Project Activity	Start Date	End Date	Total Time (weeks)
1. Recruit Team Members	2021/12/10	2022/01/10	4
2. Development of Project Plan	2023/02/02	2023/07/31	26
3. Wet Laboratory Activities	2023/08/09	2023/10/11	9
4. Dry Laboratory Activities	2023/10/07
5. Building the Team Wiki	2023/07/16	2023/10/11	12