The aim of this wet lab project was to construct a library of RBS parts for L. lactis. With enough variation in the expression of each strain with a particular sequence, we can enable fine-tuning of genes for multiple metabolic engineering goals using this organism. 

We also intended to test out computationally generated sequences based on our very own predictor-optimizer model and understand if we are able to predict expression levels reliably.

What we learned from our results is that research is always a mixed bag. We win some and lose some. When we ‘lose’, it indicates that we need to go back to the drawing board, figure out what went wrong, and try again. This cycle of design-build-test-learn happens both at the small scale of day-to-day experiments and at the large scale of testing out new hypotheses. This is illustrated clearly in our lab notebook and our engineering success.

Fluorescence Assay Results