Contribution

• Debugging BBrickIt!

Mr.Anirudh Veliyur Played a pivotal role in debugging the software BBrickIt! developed by the iGEM Team ICT Mumbai 2018:
1. Made a few grammatical and spelling corrections to the previous version.
2. def GetStringWithoutNumbers(dna1):
line=dna1
line1=[]
for i in range (len(line)-1):
base=line[i]
if base == 'a' or base =='A':
line1 += ['a']
elif base == 't' or base =='T' or base == 'u' or base == 'U':
line1 += ['t']
elif base == 'c' or base == 'C':
line1 += ['c']
elif base == 'g' or base == 'G':
line1 += ['g']
else: #Edit 2023
messagebox.showerror("Error!", "Your coding sequence has invalid characters. \nInput valid characters (A,U,G,T,C) and BioBrick again.
")
break
Included an error box to notify user of any invalid character, which is, any character other than A,U,T,G,C.
3. def clear2():
#Edit 2023 global txt4
global lbl_warn
global lbl_help
lbl_warn.pack()
lbl_warn.pack_forget()
lbl_help.pack()
lbl_help.pack_forget()
txt4.pack()
txt4.pack_forget()
txt3.place(x = 30, y = 340 , width=700, height=150)
txt3.delete("1.0","end")
lbla.configure(text="")
lbl3.configure(text="")
lbl4.configure(text="")
lbl5.configure(text="")
lbl6.configure(text="")
lbl7.configure(text="")
lbl8.configure(text="")
lbl_com.configure(text="")
lbl_com2.configure(text="")
btn2=Button(window, text="Clear output", bg="white", fg="black", command=clear2) #Edit 2023
btn2.place(x = 630, y = 540, width = 80)
btn1=Button(window, text="Clear input", bg="white", fg="black", command=clear1)
btn1.place(x = 540, y = 540, width = 80)
Included clear output and clear input buttons separately.
4. def copy(): #Edit 2023
if txt4.get("1.0", "end-1c"):
bbseq = txt4.get("1.0",END)
bbseq = str(bbseq)
window.clipboard_clear()
window.clipboard_append(bbseq)
else:
bbseq = txt3.get("1.0",END)
bbseq = str(bbseq)
# print(bbseq)
window.clipboard_clear()
window.clipboard_append(bbseq)
btn_copy = Button(window, textvariable=button_text, bg="white", fg="black", command=copy)
btn_copy.place(x = 550, y = 510, width = 150)
Updated code to copy either BioBricked, reverse BioBricked sequence or suggested sequence if the provided sequence could not be BioBricked.
5. txt3.tag_add('tag10','1.0 + 14 chars','1.0 + 22 chars') #Edit2023
txt3.tag_config('tag10', foreground="purple")
txt3.tag_add('tag11','end - 22 chars','end - 15 chars') #Edit2023
txt3.tag_config('tag11', foreground="purple")
lbl8.configure(text='NotI', foreground='purple') #Edit2023
lbl5.configure(text='SpeI', foreground='brown') #Edit2023
Included NotI in the legend and highlighted the sequences in the BioBricked and reverse BioBricked sequence. Changed color of SpeI to brown for better visibility.
6. # Copy_Button for Version 3
button_text = StringVar() def update_button_text(): #Edit 2023
if txt4.get("1.0", "end-1c"):
button_text.set("Copy suggested sequence")
else:
button_text.set("Copy BioBrick sequence")
window.after(10, update_button_text)
btn_copy = Button(window, textvariable=button_text, bg="white", fg="black", command=copy)
btn_copy.place(x = 550, y = 510, width = 150)
txt4.bind("", lambda e: update_button_text())
txt3.bind("", lambda e: update_button_text())
update_button_text()
Changed text of copy button to "Copy suggested sequence" if there is an invalid sequence.