Part Collection

    This year,we designed 38 parts.15 are basic parts and 23 are composite parts, two of the composite parts are improved from the HUBU-Wuhan team in igem2018. The parts have been tested to make sure they are reliable.

improved parts

Species name Part number Part name Part Type Length/bp Notes
Zymomonas mobilis(old) BBa_K2800020 Ptet-BsAls2-ilvC-ilvD Composite 5320
Zymomonas mobilis(new) BBa_K4666033 Ptet-BsAls2-Peno-ilvC-ilvD Composite 5506
Zymomonas mobilis BBa_K4666034 Ptet-BsAls2-Pgap-ilvC-ilvD Composite 5607

basic parts

Species name Part number Part name Part Type Length/bp Notes
Francisella novicida BBa_K4666000 dcpf1 coding 3903 Endonuclease dead version of the Francisella novicida cpf1 gene
Zymomonas mobilis BBa_K4666002 Ppdc regulatory 199 constitutive promoter Ppdc
Zymomonas mobilis BBa_K4666004 PBAD regulatory 1161 inducible promoter PBAD
Synechococcus elongatus PCC 7942 BBa_K4666010 prkA coding 1002 phosphoribulokinase
Thiobacillus denitrificans BBa_K4666006 rbc coiding 1440 Ribulose 1,5-bisphosphate carboxylase oxygenase (RuBisCO)
Synechococcus elongatus PCC 7942 BBa_K4666015 ccmK1 coding 362 CcmK1 protein
Synechococcus elongatus PCC 7942 BBa_K4666015 ccmK2 coding 362 CcmK2 protein
Synechococcus elongatus PCC 7942 BBa_K4666017 ccmO coding 983 CcmK2 protein
Synechococcus elongatus PCC 7942 BBa_K4666018 ccmL coding 315 Ccml protein
Synechococcus elongatus PCC 7942 BBa_K4666019 mNeonGreen coding 711 Green fluorescent protein gene
Synechococcus elongatus PCC 7942 BBa_K4666020 CA coding 711 Carbonic anhydrase
Synechococcus elongatus PCC 7942 BBa_K4666021 ccmM coding 2037 conserved scaffolding protein in β-carboxysome
Synechococcus elongatus PCC 7942 BBa_K4666022 ccmN coding 777 conserved scaffolding protein in β-carboxysome
Synechococcus elongatus PCC 7942 BBa_K4666023 RBS-9K RBS 25 The fluorescence intensity of RBS is 9k
Synechococcus elongatus PCC 7942 BBa_K4666030 ccmK1_mNeonGreen coding 1050 fusion protein of ccmK1 and mNeonGreen

composite parts

Species name Part number Part name Part Type Length/bp Notes
Zymomonas mobilis BBa_K4666001 Ptet-dcpf1 Composite 4591
Zymomonas mobilis BBa_K4666003 Ppdc Composite 211
Zymomonas mobilis BBa_K4666005 PBAD+RBS Composite 1173
Zymomonas mobilis BBa_K4666007 Ptet-BsAls2 Composite 2413
Zymomonas mobilis BBa_K4666008 Ppdc-BsAls2 Composite 1924
Zymomonas mobilis BBa_K4666009 PBAD-BsAls2 Composite 2886
Zymomonas mobilis BBa_K4666011 Peno-ilvC-ilvD Composite 3093
Zymomonas mobilis BBa_K4666012 Pgap-ilvC-ilvD Composite 3206
Zymomonas mobilis BBa_K4666013 Ptet-prkA Composite 1690
Zymomonas mobilis BBa_K4666014 Peno-rbc Composite 1641
Zymomonas mobilis BBa_K4666031 Ppdc-BsAls2-Peno-ilvC-ilvD Composite 5017
Zymomonas mobilis BBa_K4666032 PBAD-BsAls2-Peno-ilvC-ilvD Composite 5979
Zymomonas mobilis BBa_K4666035 Ptet-ccmK1-ccmK2-ccmO-ccmL-ccmK1_mNeonGreen Composite 3824
Zymomonas mobilis BBa_K4666036 Ptet-ccmM-rbc-ccmN Composite 4993
Zymomonas mobilis BBa_K4666037 Ptet-CA Composite 1399