Week 1-4

Recruiting members. Reading research papers and news to decide our 2023 iGEM topic.

Week 5-6

We have decided to investigate the issue of Melioidosis caused by Burkholderia pseudomallei. Brainstorming ideas in approaching the challenge. What are the aspects that we would like to improve based on traditional methods? What are some innovative ways that allow us to do so?

Week 7-8

We consulted with professors and experts, finalized our design of the project.

Week 9-10

We went to a scientific exhibition, introduced to visitors about concepts in synthetic biology and our project design. We read more papers and designed our plasmids using Snapgene.

Week 11-12

We worked on registry and promotional videos, continued to design plasmids, reached out to companies but did not get any replies…

Week 13-16

We presented our project to the school science teachers, they approved our project and we signed up for iGEM competition. We started to think about the chemicals we are going to be using in lab, and tried computer modeling Gromacs.

Week 17-20

We have finalized plasmid design and chemical list. We ordered some of them. We consulted with a CUHK professor where our project can be improved. The CUHK professor gave us safety and lab pipetting training.

Week 21-24

We have set time schedules for lab work, sorted a name list of members attending lab. We finished ordering the chemicals we need. Our secondary PI briefly informed us on basic biochem knowledge. We officially started the lab work. We did cell preparation and plasmid isolation during the lab.

Week 25-28

  • Lab: Cell preparation is done. We co-transformed PA1897 and pET plasmid in BL21. Used IPTG to induce release of CcdB. We did RE cut on pET21a(+)_qSCR and PA1897_EGFP. Results are confirmed positive by colony PCR and sequencing respectively.
  • Human practice: We have attended the first iGBA conference, communicated with different teams and companies. We learnt a lot about what we can improve in application in the real world.
  • Registry: We worked on details of part information, based on data.
  • Wiki: We did a lot of writing, artwork and design for the team wiki page.

Week 29-32

  • Human practice: We reached out to one more company - Filix Medtech to consult our project's real world value and application.
  • Wiki: We edited the details for the content. We started to write modifications in protocols and results of experiments. We finished the project description.

Week 33

  • Registry: We finalized all part information, and wrote on improved parts in wiki.
  • Team: We finished team member information in wiki and the attribution.