We originally planned to reduce the virulence of Burkholderia pseudomallei, however due to its virulent nature, it is a BSL-3 organism, we had to look for a constituent. Eventually we decided to use Pseudomonas aeruginosa, a BSL-1 organism that is similar to B. pseudomallei in experiment. Our initial plan includes testing biosensors on AHL molecules released by the pathogen B. pseudomallei, however, after serious consideration, we realized that by using innate AHL molecules, it may be hazardous to both the environment and animals. Upon accidental releasing of AHL outside of the lab, it could act as autoinducer for B. pseudomallei population and contaminate a large body of water. Absorption of innate AHL molecules in researchers could cause disease by the same rationale. Therefore we are determined to use synthetic AHL to prevent such possibilities. Lab coats and gloves are wore at all time by all members to prevent contamination and hazards. Gloves are removed before going out of the lab.
The major end users of our project are the Department of Health in Hong Kong. They can reduce the mass of pathogen contamination by using our biosensor. We aim to reduce the number of patients who are diagnosed with the disease by preventing it from occurring in the first place, as the conventional treatment therapy takes much cost and can be time consuming.