Different lengths of DNA duplex containing same number of nucleotides were designed in accordance with complementary base pairing principle. Details of the DNA sequences generated are documented as follow:
| Oligo | Sequence | Size (nucleotide) |
|---|---|---|
| Oligo 1 | TGCCGGGTATAGCGCTTAGCATCCCGACACCTCTACCTACGCGGCCCAAACCAACAGGGC | 60 |
| Oligo 2 | GCCCTGTTGGTTTGGGCCGCGTAGGTAGAGGTGTCGGGATGCTAAGCGCTATACCCGGCA | 60 |
| Oligo 3 | GGTTCCTCTTGCTTACGGTCGGCGGACTAGGCCGTACCTACCATC | 45 |
| Oligo 4 | GATGGTAGGTACGGCCTAGTCCGCCGACCGTAAGCAAGAGGAACC | 45 |
| Oligo 5 | GCTGTTATGAAATAGCGGGAGGTTAGACGTCAGGT | 35 |
| Oligo 6 | ACCTGACGTCTAACCTCCCGCTATTTCATAACAGC | 35 |
| Oligo7 | TCGTCATAGGCCGCACGAGTTAGAT | 25 |
| Oligo 8 | ATCTAACTCGTGCGGCCTATGACGA | 25 |
| Oligo 9 | TCATCACACTTGGAT | 15 |
| Oligo 10 | ATCCAAGTGTGATGA | 15 |
Besides the DNA duplex, our "Cascade Enzyme System" for PFAs degradation also involve two enzymes, namely Lignin peroxidase (LiP) and Manganese peroxidase (MnP), which were synthesized by the below two basic parts.
| Basic Part Name | Type | Description | Length (bp) |
|---|---|---|---|
| BBa_K4837000 | Protein-coding sequence | This basic part is used to synthesize Lignin peroxidase (LiP). | 2428 |
| BBa_K4837001 | Protein-coding sequence | This basic part is used to synthesize Manganese peroxidase (MnP). | 1974 |
The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized. The detail of the above basic parts could be found in the Engineering page.