DNA-Enzyme Duo for Degradation:
Using DNA duplex and enzymes for faster PFAS degradation

Poly-fluoroalkyl substances (PFAs), also named as "forever chemicals", are large complex groups of synthetic chemicals that are widely used in different consumers products because of its stability to heat, oil, water, etc. Hence, long lead time is needed for PFAs degradation. Research suggested that lignin peroxidase (LiP) and manganese peroxidase (MnP) can work together for PFAs degradation. In this project, we sought possibilities to speed up the PFAs degradation process by conjugating oligonucleotide with engineered enzymes, forming a "Cascade Enzyme System" of LiP and MnP.