The team was officially established. We held the first meeting where we met the rest of the team and some of the advisors.
Members of the experimental group learned the experimental safety guidelines and did safety training required by the laboratory. During the first week, we completed the design of all the required primers and began to design our experimental scheme.
We had our second meeting where we were introduced to the judging handbook. We discussed the next series of arrangements, such as experimental progress and promotion activities.
The human practices group and the experimental group members of the HBUT-China team held a one-day booth promotion activity in the central area of the campus, in order to promote the International Genetic Engineering Machine Design Competition and synthetic biology.
We decided to construct expression vectors such as Cas12a, sgRNA and donor and verify the feasibility of single and double plasmid systems.
Kong Xiang, Zeng Hongying and Zheng Yimeng, three members of the human practices group, had an in-depth communication with the head of Angel Yeast Company.
Brainstorming about the molecules we want to produce continued. This week, in addition to studying the construction of CRISPR expression vectors, we also studied the gene knockout pathway for synthesizing lycopene.
The instructor Yao Lan and the team members Kong Xiang, Zeng Hongying and Han Yi came to the kindergarten of Hubei University of Technology. In the process of popularizing science, there was laughter and laughter. The children have fun and learn biology at the same time.
In this week, we started to analyze the outstanding cases and discuss them in combination with our projects. At the same time, the members of the experimental group read the literature related to the project to improve the design and plan of the experiment.
In order to further promote the competition content and synthetic biology of the our team to more college students, the team held a science popularization class propaganda activity with the help of the Yanlin Science innovation platform of the College of Bioengineering and Food.
We decided to screen high-efficiency promoters, terminators, RBS and other components.
Plasmid with different skeleton of MAD7 was designed and synthesized, such as PEC-MAD7,pJYS1-MAD7, PEC-cpf1 and pJYS1-MAD7. We amplified and validated all the obtained plasmids.
The modified promoter fragment(PEC-PlacM-MAD7) was amplified by PCR and the plasmid was purified.
Members of the art group designed a lovely team LOGO and IP cultural and creative products for the project content, and Kong Xiang, the leader of the human practices group, was responsible for the purchasing affairs.
The constructed plasmids were transformed into E. coli TOP10, and the sequencing results of one of the clones were correct.
The modified replicon fragment(PEC-PlacM-PxmB-MAD7) was amplified by PCR and the plasmid was purified.
Members of the art team make posters for Hubei Synthetic Biology Open Conference.
Web group members complete the description page, wiki page validation and release wiki tool igem-uploads.
Our team members and HUBU-SKY-China members communicated online through the conference platform and shared their experiences in the competition.
The plasmid was transformed into Corynebacterium glutamicum, and the editing efficiency was calculated after color comparison and verification.The results show that our optimized promoter is successful!
The constructed plasmids were transformed into E. coli TOP10, and the sequencing results of one of the clones were correct.
Kong Xiang, Yu Siqin and Han Yi went to Hubei University and had an in-depth discussion with HUBU-SKY-China team on follow-up video cooperation.
At the invitation of Hubei University, the HBUT-China team participated in Hubei Synthetic Biology Open Conference. In the morning, the conference officially opened, and the participating teams carried out a preliminary display of the project. In the afternoon, the teams carried out a comprehensive display and more in-depth communication on the project content at the conference booth.
The plasmid was transferred into Corynebacterium glutamicum, and the editing efficiency was calculated after color comparison and verification. The results show that our optimized replicon is successful!
We designed the positions of PAM sites and front and rear chains in different skeletons, such as pEC-MAD7-TTTN, pJYS1-MAD7-TTTN, pEC-Cpf1-TTTN, pJYS1-Cpf1-TTTN. Amplification of the fragment, then cloning into plasmids.
Human group and art group members produced three funny children’s picture books. Our team hopes that through this, children can establish a preliminary understanding of biological knowledge and cultivate their interest in biology.
From July 8 to 10, 2023, HBUT-China team members Jiang Yiming, Liang Tianyi, Kong Xiang, Wang Niannian and Zeng Hongying participated in the 10th Conference of China iGEMer Community (CCiC) held at Hainan University, bringing together teams from all over the country. Our team members showed the team project content to the teachers and students in the conference with confidence and fluency, and won a lot of applause and recognition.
The constructed plasmids were transformed into E. coli TOP10, and the sequencing results of one of the clones were correct.
For the integrity of the experiment, we continued to design the positions of PAM sites and front and rear chains in different skeletons, such as pEC-MAD7-CTTN, pJYS1-MAD7-CTTN, pEC-Cpf1-CTTN, pJYS1-Cpf1-CTTN. Amplification of the fragment, then cloning into plasmids.
We designed the experiment of straw processing to obtain glucose, and prepared the materials needed for the experiment.
Our human practices group members, Xinqiu Hu and Yimeng Zheng, designed a questionnaire to understand the public’s understanding of synthetic biology, lycopene, straw treatment and utilization, further publicizing our project.
The plasmid was transferred into Corynebacterium glutamicum, and the editing efficiency was calculated after color comparison and verification.The high-quality PAM sites in the gene skeleton were obtained by comparing the results.
The straw powder was dried by removing the surface lipids with anhydrous ethanol.
in order to serve the society and contribute to the society, the human practices group members Zeng Hongying, Zheng Yimeng, Hu Xinqiu and two members of CUG-China team went to the nursing home, the elderly Service center of Shizishan Street Hongshan District, to carry out volunteer services.The activity was mainly in the form of artistic performances and popular science lectures, bringing joy to the elderly and relevant knowledge of popular science.
We used the CRISPR-MAD7(Cas12a) system to knock out large fragments, and designed plasmids that knocked out fragments of different lengths, such as 5kb, 10kb, 20kb, 25kb. Amplification of the fragment, then cloning into plasmids.
The dried straw powder was pretreated with dilute sulfuric acid, and the enzymatic solution was added for enzymatic hydrolysis, and the glucose solution was obtained by solid-liquid separation.
Our team members participated in the 2nd Synthetic Biology Competition · Innovation Competition sponsored by the Syntheic Biology Branch of the Chinese Society of Bioengineering at Shenzhen University of Technology.
The constructed plasmids were transformed into E. coli TOP10. Unfortunately, some of the clones turned out to be wrong, which means we have to rethink our design.
A biosensor analyzer was used to determine the glucose content in the glucose extract. Cryopreservation for future use.
Zeng Hongying, a member of the Human Practice group, returned to Xiangyang Zhiyuan Middle School to promote the team project and popularize synthetic biology knowledge. She encouraged the students to actively participate in various comprehensive competitions in the university stage, and cultivated their scientific research ability, learning ability and teamwork ability in the competition practice.
Some plasmids were transformed into Corynebacterium glutamate, and the editing efficiency was calculated by color comparison and verification. The redesigned plasmid was again transformed into E.coli TOP10 and verified again.
We use the CRISPR/MAD7 gene editing system to knock out single genes, such as CrtR, CrtEb, CrtYf. Amplification of the fragment, then cloning into plasmids.
Web group members completed software colonies-counter verification.
HP group members took straw treatment and utilization as the starting point, interviewed relevant enterprise personnel, government personnel, farmers, experts, etc, and obtained the understanding and thinking of many people in different industries on straw treatment and utilization.
We discussed the video script, style, and content, and decided on a production date.
Some plasmids were transformed into _Corynebacterium glutam__icum_, and the editing efficiency was calculated by color comparison and verification. Through the analysis of the results, we found the highest editing efficiency of the knockout fragment length in this system.
The constructed plasmids were transformed into E. coli TOP10. Sadly, all the plasmids we’ve built have failed. So we had to go back to the clone design.
Network member Han Yi went to Haikou Nongken Middle School to introduce iGEM and our program to the students. The students showed great interest in the competition, and Han Yi encouraged them to participate in the competition and improve their abilities in synthetic biology in the future.
We redesigned the method of constructing the plasmid, amplified the upstream and downstream two fragments, and then cloned them into the plasmid. As time is pressing, we speed up the experiment.
The constructed plasmids were transformed into E. coli TOP10. Happily, the cloned plasmid was verified by sequencing to be correct.
Plasmids were transformed into Corynebacterium glutamicum. Pink colonies appeared on the medium plate, which was amazing!
Through color comparison, a recombinant strain with good growth ability was isolated and selected from the basic culture. Glucose extracted from straw was added to shake flask culture medium, and the growth of strain was measured under this culture medium.
Our team members held charitable donation activities for cultural creative products and picture books, transforming the project into public charity for people in need of help.
The first draft of our video is almost complete, and the animation part is starting to be added. We were planning to complete the promotion video within a week.
After a certain period of time, a certain sample bacterial solution was taken and the yield of lycopene in the modified strain was measured by high performance liquid chromatography (HPLC) to evaluate the effect of experimental manipulation on lycopene synthesis.
We sold the designed cultural and creative products and picture books for charity and then contacted charity organizations to make donations.
We completed the recording of the narration part of the demo video and the editing of the animation in the video, and submitted it on time.
