Date July 11
Detail Team meeting for experiment schedule and team slogan & team logo
Date July 12
Detail LB and YPD culture solution was prepared and sent to the disinfection room for sterilization.
Basic rules of use in the laboratory were understood, PCR preparation was carried out, and Escherichia coli
and yeast were inoculated.
Date July 13
Detail Gel electrophoresis, spot sample, electrophoresis and blue light observation were performed. Yeast
receptive cells were prepared in the early stage, followed by yeast expansion culture. The plasmid pYES2 was
extracted and the concentration was detected after the plasmid extraction process. Clean and recover
An_phy33 gene, and test the concentration after the glue recovery process. An_phy33 and pYES2 plasmids were
studied.
Date July 14
Detail Used ligase to reconstruct the gene fragment AppA and An_phy33 with the plasmid pYES2. prepared
competent yeast cell. transformed the reconstructed plasmid into competent cell E. coli.
Date July 15
Detail Plasmid extration. Polymerase chain reaction. Agarose gel electrophoresis. Plasmid transformation.
Date July 16
Detail The plasmid cultured in E. coli was extracted, PCR and agarose gel electrophoresis were used to
confirm whether the culture was successful. Transform yeast receptor cells and apply.
Date July 17
Detail The yeast transformation strain carrying the specified plasmid was selected, inoculated with 4 mL
plus hygromycin into a 30 ℃ shaking table, and prepared with 800 mL NaAc solution with pH 5.0. Pre-configure
a solution with a pH of 1-8.
Date July 18
Detail Make albumen glue. Write wiki.
Date July 19
Detail Extract yeast cells then shake them up.
Date July 20
Detail Upload basic parts information and write wiki.
Date July 21
Detail One day off
Date July 22
Detail Run SDS PAGE gel.
Date July 23
Detail Phytase activity test by microplate reader
