During the progress of iGEM, we had many different experimental setups to test and research all of our individual parts. For reocurring procedures, we collected and adapted many general protocols for various daily lab tasks. On this page, all general protocols are listed. These protocols were improved by us to fit our needs and setups and were used in all aspects of our lab work.
Standard protocols to create media and making the corresponding plates
Standard protocols for antibiotic-stocks and storage conditions
Cloning and Transformation Protocols
Standard protocols for each different cloning, transformation, PCR-set up and DNA preparation
SDS and Western-Blot
Standard protocols for buffer preparation