Parts

Overview

Our team used a variety of different parts by creating new primers, using plasmids and parts from other research papers, and altering our current parts. Our team contributed a total of 31 different parts.

Parts Table

Name Type Description Designers Length
BBa_K4651020 Primer Forward primer for MucK David Lanster and Grace Wang 54
BBa_K4651021 Primer Reverse primer for MucK David Lanster and Grace Wang 42
BBa_K4651022 Primer Forward primer for rest of MucK plasmid David Lanster and Grace Wang 37
BBa_K4651023 Primer Reverse primer to linearize IPTG-inducible construct for cloning in MucK David Lanster and Grace Wang 36
BBa_K4651025 Primer Forward primer to alter -10 and -35 sigma factor on Tpar-Regulated Promoter to match pTPA3 David Lanster and Jason Gao 20
BBa_K4651025 Primer Reverse primer to alter -10 and -35 sigma factor on Tpar-Regulated Promoter to match pTPA3 David Lanster and Jason Gao 60
BBa_K4651026 Primer Forward primer that truncates 3’ end of original tpaA-tpaR intergenic, bi-directional promoter to remove endogenous RBS David Lanster and Jason Gao 38
BBa_K4651027 Primer Reverse primer that truncates 3’ end of original tpaA-tpaR intergenic, bi-directional promoter to remove endogenous RBS David Lanster and Jason Gao 20
BBa_K4651028 Primer Forward primer to linearize pIGEM1 (tpaR plasmid) David Lanster 29
BBa_K4651029 Primer Reverse primer for to linearize pIGEM1 (tpaR plasmid) David Lanster and Jason Gao 37
BBa_K4651002 Reporter Creates scarlet fluorescent signal Ahmed Badran 696
BBa_K4651030 Primer Forward primer for pTPA3, anneals to DL1041 David Lanster and Jason Gao 60
BBa_K4651031 Primer Reverse primer for pTPA3, anneals to DL1040 David Lanster and Jason Gao 60
BBa_K4651001 Reporter Creates green fluorescent signal Ex: 487 nm/Em: 509 nm Ahmed Badran 717
BBa_K4651003 Antibiotic Resistance Marker Antibiotic resistance marker for carbenicillin Badran lab collection 861
BBa_K4651004 Promoter Drives expression for bla (AmpR) antibiotic resistance marker Badran lab collection 195
BBa_K4651005 Origin of replication Works together with pSC101 to make copies of plasmid Badran lab collection 951
BBa_K4651006 Origin of replication Works together with RepA to make copies of plasmid Badran lab collection 746
BBa_K4651007 Promoter Drives expression of mEmerald or mScarlet, and TpaR David Lanster and Ahmed Badran taken from reference 2 164
BBa_K4651008 Transcription Factor Starts transcription for both plasmids David Lanster and Ahmed Badran taken from reference 2 735
BBa_K4651009 Terminator Ends transcription Badran lab collection 432
BBa_K4651010 Importer Imports TPA into plasmid Ahmed Badran taken from reference 2 1380
BBa_K4651011 Importer Imports TPA into plasmid Ahmed Badran, taken from reference 1 1242
BBa_K4651012 Antibiotic Resistance Marker Antibiotic resistance marker for spectinomycin Badran lab collection 792
BBa_K4651013 Promoter Drives expression of MucK and TpaK Badran lab collection 139
BBa_K4651014 Origin of replication Works with ColE1 to make copies of plasmid Badran lab collection 192
BBa_K4651015 Origin of replication Works with Rop to make copies of plasmid Badran lab collection 589
BBa_K4651016 Protein Protein that controls MucK Badran lab collection 1083
BBa_K4651017 Promoter Promoter taken from reference 1 David Lanster, taken from reference 1 111
BBa_K4651018 Promoter Modified TpaR-Regulated Promoter to be truncated to remove native RBS David Lanster and Jason Gao 153
BBa_K4651019 Promoter Modified TpaR-Regulated Promoter to have -10 and -35 sigma factor regions changed to match pTPA3 David Lanster and Jason Gao 163

References

[1] Pardo I, Jha RK, Bermel RE, Bratti F, Gaddis M, McIntyre E, Michener W, Neidle EL, Dale T, Beckham GT, Johnson CW. Gene amplification, laboratory evolution, and biosensor screening reveal MucK as a terephthalic acid transporter in Acinetobacter baylyi ADP1. Metab Eng. 2020 Nov;62:260-274. doi: 10.1016/j.ymben.2020.09.009. Epub 2020 Oct 1. PMID: 32979486.

[2] Hara H, Eltis LD, Davies JE, Mohn WW. Transcriptomic analysis reveals a bifurcated terephthalate degradation pathway in Rhodococcus sp. strain RHA1. J Bacteriol. 2007 Mar;189(5):1641-7. doi: 10.1128/JB.01322-06. Epub 2006 Dec 1. PMID: 17142403; PMCID: PMC1855752.