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Basic Parts

We have designed 12 basic parts this year. They are mostly indispensable in our loop. And we use the method of homologous recombination to construct plasmids using these basic parts, which is convenient and efficient.

Basic Parts
Name Type Description Designer Length
BBa_K4585000 Coding GnRH-C  Siyao Ma 99bp
BBa_K4585001 Coding GAL4 Siyao Ma 438bp
BBa_K4585002 Coding VP64 Siyao Ma 150bp
BBa_K4585003 Coding KRAB  Siyao Ma 186bp
BBa_K4585004 DNA 9XUAS Siyao Ma 182bp
BBa_K4585005 Coding Degron Zijun Zhang 126bp
BBa_K4585006 Composite pcDNA3.1(+)-3×HA-Gal4-VP64-NLS linearized vector Siyao Ma 6038bp
BBa_K4585007 Composite pcDNA3.1(+)-3×HA-Gal4-KRAB-NLS linearized vector Siyao Ma 5600bp
BBa_K4585008 Composite pGL4.35-3×HA-9×GAL4UAS-KRAB-NLS linearized Siyao Ma 4500bp
BBa_K4585009 Composite pcDNA3.1(+)-3×HA-Gal4-VP64-Degron-NLS linearized vector Zijun Zhang 6237bp
BBa_K4585010 Composite pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS linearized vector Zijun Zhang 5338bp
BBa_K4585016 Composite pcDNA3.1(+)-GnRH-C linearized vector Siyao Ma 5346bp

Compisite parts

The plasmids we used were mainly used in two experiments, the first for detection of changes in intracellular calcium flow and the other for intracellular rhythm detection experiments. The main way for the plasmid construction is homologous recombination experiment with the help of PCR experiment, and the target product is obtained by reaction the target fragment and the corresponding linear vector to the proportion.The plasmids obtained by this method were applied to the experiments after sequence detection.

Basic Parts
Name Type Description Designer Length
BBa_K4585011 Plasmid pcDNA3.1(+)-GnRH-C Siyao Ma 5445 bp
BBa_K4585012 Plasmid pcDNA3.1(+)-3×HA-GAL4-VP64-NLS Siyao Ma 6118 bp
BBa_K4585013 Plasmid pGL4.35-3×HA-9×GAL4UAS-KRAB-NLS Siyao Ma 5296 bp
BBa_K4585014 Plasmid pcDNA3.1(+)-3×HA-GAL4-VP64-Degron-NLS Zijun Zhang  6326 bp
BBa_K4585015 Plasmid pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS Zijun Zhang  5434 bp

Our Sponsors 2023

We proudly thank all our sponsors.
Without their support we would not have been able to achieve this project.

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