Wet Lab Notebook

2023.6.20

Order Experiment Materials

2023.7.1

Online Meetup for determining lab schedules

2023.7.6

1.  Project Design Review

2.  Lab Safety Training

2023.7.7

1.Configure the culture medium
2.Extract plasmids
3.digestion
4.Switch

2023.7.8

1.Agarose gel electrophoresis
2.Rubber recycling
3.T4 DNA Enzyme connection
4.Conversion (DH5α) Conversion BL21

2023.7.9

1.Pick single clone, shake bacteria
2.Make protein glue
3.plasmid extract
4.digestion

2023.7.10

Group A: plasmid extract, enzyme cutting,Agarose gel electrophoresis
Group B: Sample pretreatment

2023.7.11

Send for sequencing

2023.7.1 2

1.Expanded training (need to start from 9 o’clock)
2.Sort out the SDS-PAGE results to determine the best expression conditions
3.Add IPTG
4.Collect bacteria

2023.7.1 3

1.Collect protein
2.Pretreatment of dialysis bag (estimated to take 30min)
3.Contains body complex (dialysis)

2023.7.1 4

1.Nickel column purification
2.BCA Quantitative
3.SDS-PAGE Glue to detect protein purity

2023.7.1 5

Utilize ELISA examine SARS-CoV-2 RBD with people ACE2 the binding ability of protein

2023.7.1 6

Data curating and analysis