As can be seen in the introduction our product is an ELISA kit with a special kind of antibody. So we first design the plasmids for the protein by homologous recombination. After a series of steps such as transfection, we successfully obtained the desired antibody. To test it, we coated the  antibody on a 96-well plate as a tool antibody for ELISA detection. Fortunately, our antibody is successful and we attained a standard curve for our ELISA kit.

 

Week 1

Did the ice breaking activities and determined our team name and logo. Learned about our project and allocated jobs to different teamates.

 

Week 1 Visited the lab. Prepared the LB broth and agarose electrophoresis gel. Prepared the gel for SDS-PAGE, did the cell resuscitation.

 

Week 2

Digested the plasmid base.Did the polymerase chain reaction. Constructed the plasmids by homologous recombination. Tested the plasmid through agaros electrophoresis gel then purified the gel.

 

Week 2

Incited the monoclonal culture and waited for results for experiment we did before. Communicated with dry lab about the future collavoration.Picked up monoclone from petri dish

 

Week 3

Reanimate the cell that we need for the transfection. Transfected the plasmid into cells to make them combine inside cells. Transfered the cells into a erlenmeyer flask to make them expess the antibody we want.

 

Week 3

Purified the protein that expressed from the cell which is introduced with plasmids. Analyzed the SDS-PAGE gel.

 

Week 4

Did the ELISA test to make sure that our antibody is effective. Make the standard curve for our product.